| Objective To investigate the expression of Pak1, LIMK1 and Cofilin-1 proteins in gastric mucosa beside carcinoma, precancerous lesion and gastric carcinoma tissues, and to research the correlation between the three things after taking clinical manifestations and pathological results into consideration, and to detect their effect during the development of gastric cancer and the process of tumor invasion and metastasis and find the possible mechanism of basil polysaccharide in inhibiting tumor metastasis.Methods Collecting 40 cases gastric adenocarcinoma, 30 cases precancerous lesions, and 30 cases gastric mucosa beside carcinoma tissue after surgery, SABC immunohistochemistry methods was adopted to investigate the changes of Pak1, LIMK1 and Cofilin-1 proteins expression in different cases, and to analyze its correlation with the gender, age, tumor size, differentiated degree, lymphatic metastasis and TNM stages of gastric carcinoma patients.Results1. In gastric cancer tissues specimen, the positive expression rate of Pak1 protein existed in gastric cancer tissues, precancerous lesions tissues and normal gastric mucosa specimens was respectively 85.0%,50%,20%, which had a significant difference among them(P<0.05). The positive expression rate of LIMK1 protein existed in gastric cancer tissues, precancerous lesions tissues and normal gastric mucosa specimens was87.5%,53.5%,23.3%, which had a significant difference among them(P<0.05), The positive expression rate of Colifin-1 protein existed in gastric cancer tissues, precancerous lesions tissues and normal gastric mucosa specimens was 80%,56.7%,16.7%, which had a significant difference among them(P<0.05).2. As show by immunohistochemistry, the positive expression rate of Pak1 protein was in the TNM stage III and IV, lymph node metastasis group and lower tumor differentiation were higher significantly than that of non-lymphatic metastasis group and middle to well differentiated in the TNM stage I and II(P<0.05). The positive expression rate of LIMK1 protein was in tumor ≥3cm and the TNM stage III and IV, lymph node metastasis group and lower tumor differentiation were higher significantly than that of non-lymphatic metastasis group and middle to well differentiated in tumor <3cm and the TNM stage I and II(P<0.05). The positive expression rate of Cofilin-1 protein was in the TNM stage III and IV, lymph node metastasis group and lower tumor differentiation were higher significantly than that of non-lymphatic metastasis group and middle to well differentiated in and the TNM stage I and II(P<0.05). The positiveexpression rate of Pak1 and Cofilin-1 in gastric cancer tissues had no difference among patient’s age, sex and tumor size, while the positive expression rate of LIMK-1 had no difference between patient’s age and sex.3. 3. By bivariate correlation analysis, it is found that the expression of Pak1 protein and LIMK1 protein in gastric cancer had linear positive correlation, while the expression with Cofilin-1 protein had negative correlation.Conclusion 1.Pak1, LIMK1 and Cofilin-1 proteins were over expression in gastric cancer carcinoma, which may be an early event in gastric carcinogenesis. 2. The expression of Pak1 and Cofilin-1 proteins correlated with lymphatic metastasis, histological differentiation and TNM stages, which may closely associated with gastric cancer occurrence, invasion and metastasis. |
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