A Study Of NPC2 Protein Interaction With PARK9 F1 Region Protein

Niemann-Pick disease(N iemann-Pick type C, NPC) is an autosomal recessive genetic disease. C urrently, NPC is considered as an unique degenerative disease caused by intracellular and extracellular cholesterol and lipid transport disorders. 5% of NPC disease is caused by the lysosomal protein NPC2 mutations which can binding with cholesterol soluble. PARK9 is the pathogenic gene of autosomal recessive inheritance for adolescent adult Parkinson’s disease(also called kufor-Rakeb), also known as ATP13A2.O ur previous experiments’ data showed that NPC2 maybe had interaction relationship with PARK9.The encoded PARK9 protein may be involved in the regulation of cell material transport within the activities.In this study we applied computer simulations、 the technology of in vitro recombination to express PARK9 fragments and NPC2 full length proteins in vitro, and successfully obtained the corresponding protein; Silver staining and GST pull-down method indicated that there was interaction relationship between NPC2 and PARK9; Dot blot experiments preliminary studied the quantum relationship between the two protein, When the concentration was close to 1:1, NPC2’s volume with PARK9 F1’s volume between 1:4-1:1 was better to combine. The subject of study focused on the interaction between NPC2 and PARK9 in order to reveal PARK9 proteins can be combined with NPC2 involved in regulation of dissolving enzyme cholesterol transport.