Effects Of Different Intensity Swimming Exercise On Morphology And Apoptosis In Rat Liver

Objective:Apoptosis of concern within the scope of human movement science, this experiment reveals the relationship between exercise intensity at different intensities between apoptosis and liver of rats, conducted from morphology and apoptosis detection of both Discussion for the prevention and treatment of sports -related liver damage has important practical significance, but also provide theoretical support for scientific sports training and sports teaching.Methods:The 3-month-old healthy male SD rats were randomly divided into three groups:sedentary control group 10, aerobic exercise group 8 and excessive exercise group 8. Sedentary control group was not swimming, aerobic exercise group and excessive exercise group reported the reference method Tezini GC, Hu yazhe and improved preparation of animal swimming and other sports model:a period of 10 weeks of training, training six times a week, rest one day, the first adaptive swim two weeks (no load). After the adaptive training, aerobic exercise group rats were not forced to swim training 60min/d. Rats were exposed to excessive weight-bearing exercise group swimming 120min/d, the first three weeks of negative weight is about 1% of body weight, the first four weeks is about 2% of body weight, the first five weeks of about 3% of body weight. Load the first 6-10 weeks motion is about 6% of body weight prolonged swimming was 150min/d. All three groups of rats were anesthetized open the abdominal cavity after the liver tissue, paraffin cuts preparation for HE staining and hepatocyte apoptosis (TUNEL) detection.Results and analysis:(1) HE staining of liver cells showed:sedentary group structure intact liver cells, red staining cytoplasm clear, a clear expression of hepatic cords, large and rounded nuclei; aerobic exercise group hepatocytes gradually changing contours, red staining cytoplasm slightly confusing, intercellular and quiet compared to the control group was significantly larger; excessive motion blur structure in liver cells, arranged in the cytoplasm loose deformation, hepatic cord irregular nucleus becomes small, cloudy swelling, and some watery degeneration. (2) sedentary group can occasionally see the nucleus, aerobic exercise group expression appeared more positive expression in the nucleus, excessive exercise group had more dark brown brown positive cells; compared with sedentary control group aerobic exercise group and excessiveexercise group showed IOD scores highly significant difference (p<0.01): compared with the aerobic exercise group, excessive exercise group IOD scores showed a very significant difference (p<0.01); among the three groups, IOD highest score over the exercise group.Conclusion:1. a control group, and the quiet and the aerobic exercise group compared to the influence of excessive exercise on liver morphology of apoptosis is more evident, indicating that excessive movement of the organizational structure of the liver cell damage is more severe, can lead to liver cells irreversible pathological changes; 2. excessive exercise on apoptosis of liver expression of the largest, followed by aerobic exercise, which is observed in liver tissue structure and morphology consistent with the results, you can guess:the number of apoptotic cells when the liver is also excessive exercise increased, which will result in deletion of liver function in rats when the body has yet to repair damaged tissue, and the emergence of new excessive stimulation, if such damage is sustained long-term accumulation, then, together with other incentives to cause of liver-related movement disorders.