The Expression Of SLC39A6Protein In Esophageal Squamous Cell Carcinoma And Clinical Significant

Objective: In esophageal squamous cell carcinomas, low level intraepithelial neoplasia, high levelintraepithelial neoplasia and normal esophageal mucosa tissues detection SLC39A6encoding proteinexpression, and analyze the SLC39A6significance in ESCC its prognostic significance..Method: In this study, SLC39A6was detected by immunohistochemistry (IHC) using tissue microarraysfrom two validation cohorts,142Han ethnic ESCC patients and86Kazakh ESCC patients, of which228(142Han,86Kazakh) were from primary cancer sites,133(Han) precursor lesions from adjacentmucosa and173(132Han,41Kazakh) from distant normal mucosa. To compare differences indemographic and clinical factors between the two validation cohorts, t test was used for continuousvariables, and χ2test was used for categorical variables. Differences in SLC39A6expression and clinicalcharacteristics were compared by χ2test. Correlations between prognostic outcomes and with SLC39A6expression were investigated using Kaplan–Meier analysis and the Cox proportional hazards model.Results: The overall rate of SLC39A6overexpression(IS＞4) in nomal samples was14.39%, low-gradeintraepithelial(LGESIN) was51.85%, high-grade intraepithelial neoplasia(HGESIN) was76.92%, in Hanethnic ESCC was69.01%. And SLC39A6was also overexpressed in Kazakh ESCC (P＜0.05). In addition,SLC39A6expression inversely correlated with histological grade (P＜0.05) and lymph node metastasis (p<0.05). Furthermore, ESCC patients having overexpressed SLC39A6experienced worse overall survival(OS) than those having low levels of SLC39A6(P=0.009). SLC39A6protein expression in esophagealcancer age, sex, clinical TNM stages was statisticaled no significant (P>0.05). SLC39A6overexpressionwas determined to be an independent prognostic factor for poor OS in ESCC patients(HR：2.651,95%CI：1.248–5.629, P=0.011). And SLC39A6was also overexpression in Kazakh ethnic ESCC.Conclusion: SLC39A6is highly expressed in ESCC and its precursor lesions, correlations withdifferentiation and lymph node metastasis. Patients with SLC39A6overexpression experienced a worseOS than those with a weak or negative SLC39A6expression. SLC39A6could serve as an independentbiomarker predicting poor prognosis in ESCC patients.