| objective: Colorectal cancer is one of the most commonmalignant tumors in gastrointestinal tract. Though the diagnosis andtreatment have been largely developed, the5-year survival rate is stillunder40%. The primary cause of death in colorectal cancer is the tumormetastasis and recurrence. Along with the development of molecularbiology, finding the invasion-and-metastasis-related marker in colorectalcancer has been highlighted, which is significant for the diagnosis, clinictreatment and prognosis evaluation. Here, we investigated the expressionof CK20, Nm23and Ki67in the epithelium of tumor and normal tissuesfrom92colorectal cancer patients and detected the relationship betweenthe3tumor markers and clinic pathology. We wished to find efficientbiological marker(s) for early diagnosis and in colorectal cancer.Methods: Resected tumor and adjacent normal epithelium from92colorectal cancer patients in enterochirurgia, affiliated hospital, LuzhouMedical college (In February2013to November2013) were collected forthe detection of CK20, Nm23and Ki67expressions using SP method.The relationship between the expressions of CK20, Nm23and Ki67andclinic pathology was analyzed. Upon the expression levels of CK20,Nm23and Ki67in each group, we analyzed the significance of CK20,Nm23and Ki67expressions in colorectal cancer. Results:1．In the92specimens, we failed to detect CK20in normal epithelium while62tumor epithelium expressed CK20(67.4%). Eighty-three normal epitheliumexpressed Nm23(90.2%) and47tumor epithelium expressed CK20(51.1%). Hyper-expression of Ki67was detected only in1normalepithelium (1.1%) and it was in59tumor epithelium (64.1%). Thecomparisons in the CK20, Nm23and Ki67expressions between normalepithelium and tumor epithelium were all statistically significant(P<0.05).2．The CK20expression was significantly related with tumorinfiltration, differentiation, lymphatic node metastasis and Dukes staging(P<0.05). The expression level of CK20was positively related withtumor infiltration while negatively related with tumor differentiation. Inaddition, the CK20expression was not related with sex, age, distantmetastasis, tumor size and site (P>0.05).3．The Nm23expression wassignificantly related with tumor infiltration, lymphatic node metastasis,distant metastasis and Dukes staging (P<0.05). The expression level ofCK20was negatively related with tumor infiltration. The Nm23expression was not related with sex, age, differentiation, tumor size andsite (P>0.05).4．The Ki67hyper-expression was significantly relatedwith tumor infiltration, lymphatic node metastasis and Dukes staging(P<0.05). The expression level of Ki67was positively related with tumorinfiltration. While, the Ki67expression was not related with sex, age,differentiation, distant metastasis, tumor size and site (P>0.05).5．CK20was negatively related with Nm23in colorectal tumor analyzed bySpearman (r=-0.588, P=0.000). Nm23was negatively related with Ki67 in colorectal tumor (r=-0.324, P=0.002). CK20was positively relatedwith Ki67in colorectal tumor (r=0.592, P=0.000). Conclusion:1．CK20is not expressed in normal epithelium and it is positively related withtumor infiltration, differentiation, regional lymphatic node metastasis andclinic staging in colorectal cancer. Therefore, CK20is considered as oneof the favorable markers for the prognosis evaluation and micrometastasisdetection.2．Nm23expression is positively related with tumor infiltration,metastasis and clinic staging in colorectal cancer. Nm23could reflecttumor growth and metastasis and could be a marker for invasive capacityof colorectal cancer.3．Ki67expression is positively related with tumorinfiltration, lymphatic node metastasis and clinic staging in colorectalcancer. Ki67could also be implicated in tumor growth; therefore, Ki67could be a marker for invasion of colorectal cancer and prognosisevaluation as well.4．The expression level of CK20is negatively relatedwith Nm23; and the expression level of Nm23is negatively related withKi67, indicating a potential interaction among the3proteins in thedevelopment of colorectal cancer. Nm23and Ki67could be new targetsin cell cycle regulation to inhibit colorectal cancer cell proliferation andinvasion, which might be provide a new clue for the biological treatmentin colorectal cancer. |
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