The Mechanism Of Acupuncture To The Kidney Injury In Rhabdomyolysis-induced Acute Renal Failure In Rats

objective: To observe the acupuncture in kidney tissue frominjury of acute renal failure (ARF) in rhabdomyolysis-induced rats.Methods： The animal model of rhabdomyolysis-induced acute kidneyinjury induced by injected glycerol in hind legs muscle.SD rats weredivided into normal control group (NC-G), ARF group (ARF-G),Acupuncture group (AC-G). Two sub-groups were divided inSynchronization acupuncture group (SA-G) and Acupuncture-pretreatment group (AP-G) in advance. The renal function, creatine kinase,kidney histopathology, k+and bax, bcl-2were investigated. Results：Experimental research ARF group acupuncture group and both kidneyswere significantly larger, edema in rats, soft, deep purple-black color, morepale section of renal cortex, Medulla, dark red. ARF groups HE voiddegeneration of pigmented cells edema, bureaucratic Myoglobin tube.Acupuncture group pathology ARF groups light model of potassium ions issignificantly higher than normal control groups had significant statistically(P<0.01)， Synchronize the acupuncture group and potassium ions issignificantly higher than normal control group was statistically significant(P<0.05), the acupuncture group ahead of potassium ions is significantlyhigher than the normal control group was statistically significant (P<0.05),and the synchronization of the acupuncture can significantly reduce themodel group of potassium ions have statistically significantly (P<0.01), theadvance group of acupuncture can significantly reduce the potassium ion ofmodel groups with significant statistical significance (P<0.01）.Modelgroup of phosphocreatine kinase (CK-C) is higher than the normal controlgroup was statistically significant (P<0.05), the group can be significantlyreduced by acupuncture model groups in advance of phosphocreatine kinase (CK-C) was statistically significant (P<0.05). ATN scores showmodel group is higher than the normal control group was statisticallysignificant (P<0.05), and the synchronization of the acupuncture cansignificantly reduce the model group ATN scores was statisticallysignificant (P<0.05), early acupuncture group can significantly reduceATN scoring statistical significance of the model group (P<0.05). Modelgroup, serum creatinine (Scr) have significant statistically significantlyhigher than the control group (P<0.01), the synchronized group ofacupuncture serum creatinine (Scr) have significant statisticallysignificantly higher than the control group (P<0.01), ahead of group ofacupuncture serum creatinine (Scr) compared with normal control groupwas not statistically significant (P>0.05), And the synchronization of theacupuncture can significantly reduce the model group serum creatinine (Scr)was statistically significant (P<0.05), early acupuncture group cansignificantly lower serum creatinine of model groups (Scr) had significantstatistically (P<0.01), early acupuncture group can significantly reducesynchronization group of acupuncture serum creatinine (Scr) wasstatistically significant P<0.05). Model group, blood urea nitrogen (BUN)have significant statistically significantly higher than the control group(P<0.01), synchronize the acupuncture group blood urea nitrogen (BUN)have significant statistically significantly higher than the control group(P<0.01), the acupuncture group ahead of blood urea nitrogen (BUN) andnormal control group was not statistically significant (P<0.01),Acupuncture group and synchronization for model groups of blood ureanitrogen (BUN) was statistically significant (P<0.05), early acupuncturegroup can significantly reduce the model group of the blood urea nitrogen(BUN) has significant statistically (P<0.01), early acupuncture acupuncturecan significantly reduce synchronization group group of blood urea nitrogen (BUN) was statistically significant (P<0.05). Model group of renaltubular epithelial injury in Bax expression than blank group statisticallysignificantly(P<0.01) and the synchronization group for the model ofacupuncture group was statistically significantly reduced Bax expression(P<0.01), the acupuncture group ahead for model groups can significantlyreduce Bax expression have a significant set of statistical significance ofthe model (P<0.01). Bcl-2/Bax ratio of a model group have significantstatistically significantly higher than the control group (P<0.05) and thesynchronization Bcl-2/Bax acupuncture group for model groups cansignificantly reduce a statistically significant odds ratio (P<0.05), theacupuncture group ahead for model groups can significantly reduce modelBcl-2/Bax ratio has a significant set of statistical significance (P<0.01).Acupuncture group compared with the ARF group has a protective effecton renal tubular Bcl-2expression.Conclusion：1.Acupuncture may relieverhabdomyolysis caused by pathological changes in kidney tissue of SD ratswith acute renal failure2. Acupuncture reduces rhabdomyolysis of SD ratmodel of acute renal failure induced by Scr, k+, BUN, CK-C3.Acupuncture can reduce the kidney injury and regulate the expression ofbcl-2, bax in SD rats of rhabdomyolysis-in-duced acute renal failure.