The Protective Effect On Intestine Of Propofol After Hemorrhagic And Resuscation In Rats

ObjectiveEarly fluid resuscitation is the main treatment for hemorrhagic shock. Improved intestinal microcirculation ultimately depends on the improvement of general conditions and adequate systemic blood volume, Active fluid resuscitation is the best way to avoid the intestinal microcirculation dysfunction. However, after general fluid resuscitation, Part of the microvascular endothelial cell swelling, Leukocyte adhesion impaction lead to Microvascular no-reflow phenomenon, a number of organizations that continue to exist ischemic after resuscitation. In response to these phenomena, in recent years, also proposed new ideas, such as recovery or delayed recovery in small doses, but the effect is still not satisfactory, so in the course of the treatment of hemorrhagic shock is no longer confined to the basic treatment of resuscitation, for the recovery process and recovery after ischemia-reperfusion injury is more and more attention, thus people have made a series of theory, a number of studies that aim directly at possible mechanisms and put forward a number of prevention methods, A pretreament, drug therapy, the traditional medicine therapy and some therapies on the level of gene and cell. Propofol as a pretreatment or throughout the whole course of treatment of hemorrhagic shock drug, in reducing intestinal injury has been a large number of literature reports, however, for hemorrhagic shock and resuscitation has been emergence of intestinal ischemia-reperfusion injury, the use of propofol would still play a protective effect on intestinal tissue, not been reported, To determine the protective effect on intestine of propofol after hemorrhagic shock and resuscitation in short-term.MethodTwenty-four Wistar rats were randomized into3groups:sham operation group (C group), resuscitation group(HS-R group) and propofol group(P group). There were8rats in each group. C group to which the internal jugular vein cannulation,carotid artery cannulation and endotracheal intubation, do not be bleeding and transfusion intervention; HS-R group, P group established animal model of hemorrhagic shock and resuscitation. Following resuscitation, the resuscitation group was infused normal saline at lml/kg/h with infusion pump for2hours, the propofol group was infused propofol at lml/kg/h for2hours. Then sampling in each group.Taken blood samples of carotid, measuring plasma diamine oxidase(DAO) level by Colorimetric method, taken5cm intestinal tissue, which measured3cm, measuring hepatocuprine(SOD)^melondialdehyde(MDA) levels respectively by xanthinoxidase method and thiobarbituric acid colorimetric technique, another2cm to do paraffin sections of intestinal tissue, measured by immunohistochemical staining of intestinal tissue BCL-2/BAX protein level, using TUNELL method to measure the level of intestinal cell apoptosis and do HE staining to observe the changes of intestinal pathological histology.ResultsThe MDA and DAO levels in the HS-R group and the P group were higher than those in the C group (P<0.5), P group was lower than HS-R group(P<0.5); but SOD levels were lower than those in the C group (P<0.5), P group was higher than HS-R group(P<0.5); HS-R group and P group BCL2protein levels lower than those in C group, P group was higher than HS-R group (all P<0.05); the expression of BAX protein in the HS-R group and P group were higher than, P group was lower than HS-R group (all P<0.05); HS-R group and P group intestinal tissue apoptosis index higher than those in C group, P group was lower than HS-R group (all P<0.05); The pathological grades of intestine in the P group and the HS-R group were higher than in the C group(P<0.05),but P group was lower than HS-R group(P<0.05).ConclusionThe intestinal tissue was significantly damaged after hemorrhagic shock and resuscitation, However, compare continuous infusion of propofol group to a simple recovery group, can observe that in the treated group directly response to the damage level of tissue injury indicator the DAO levels in plasma was lower than the simple recovery group, the MDA level in intestinal tissue that can cause tissue oxygen free radicals damage was also lower than the simple recovery group, but the SOD level in intestinal tissue that can antioxidant free radical damage was higher than the simple recovery group; the Bax protein that can lead to apoptosis and intestinal apoptosis index were lower, while the anti-apoptotic BCL-2protein levels was higher than the simple recovery group. Observations on the above indicators, propofol can through antioxidant free radical damage and regulation the balance of apoptosis and anti-apoptotic proteins to reduce intestinal tissue injury, to a certain extent to protect the integrity of the intestinal tissue mucosa and thus play a protective role function in a short time, the protective effect on intestine of propofol in longer-term needs futher research.