| Objective: To understand more about EBV-positive diffuse large B-cell lymphoma pathogenesis andclinicopathological significance through examining infection with EB virus and p100/p52proteinexpression in diffuse large B-cell lymphoma in XinJiang Autonomous Region, And To investigate therelationship between EBV infection of diffuse large B-cell lymphoma and expression of p100/p52protein.Methods: Collecting the people’s Hospital of Xinjiang Uygur Autonomous Region from2008to2013inbetween the diagnosis of diffuse large B-cell lymphoma of the Uygur, Han patients with clinical data, theselection of the relevant pathology and paraffin blocks; panel of Phenotypic maker (CD20, CD3, CD10,bcl-6, MUM-1) were performed using formalin fixed paraffin-embedded tissue sections to undiagnosedpatient. The standard according to the2008version lymphatic and hematopoietic tumors WHO newclassification by high qualification and experienced pathologist combined with morphological andimmunological phenotypes rediagnosis and histological type. To choose the190cases of patients who metthe study condition, using in situ hybridization and immunohistochemical staining methods detect the largeor medium-large cells in DLBCL EBV-encoded mRNA (EBER-1), and immuohistochemistry was used todetect the expression of p100/p52protein.Results: There were190cases of DLBCL including Uygur78cases accounted for41.05%and112casesof Han, Han is higher than the incidence of Uighur. Uighur in52males,26females, and the ratio of male tofemale is2:1; Han male to female ratio was1.38:1, Han Uighur were above normal incidence of male. Thepositive rate of EBER-1in the190case of DLBCL was12.63%, among nine cases accounted for11.54%of patients Uygur, Han15cases. Male to female ratio of3:1;24cases of EBV-positive DLBCL patientsolder than40years, the average incidence age of70.04years, the peak incidence of the Han Age70~yearsold,60~years old Uighur as Han onset patients older than Uighur. EBV positive DLBCL in Uygur andHan comparison groups, age (70for the sector) and International Prognostic Index of the difference wasstatistically significant (P<0.05), IPI Han in low-risk patients than the Uighur; no significant difference inother clinical and biological indicators. DLBCL in EBV infection group were compared with thoseuninfected, IPI and LDH was found statistically significant in both (P<0.05), which is the prognosis maybe a factor. The expression of p100/p52Protein located in medium-sized or large cell nucleus andcytoplasm, the total positive expression rate of47.33%,24cases of EBV positive DLBCL in22cases ofexpressed. There was the correlation between the p100/p52protein and EBER-1(r=0.361, P<0.05).Conclusions: DLBCL might be related to EBV latent infection and be considered as potential markers toidentify in diagnosis.(1)DLBCL of Xinjiang region was associated with EBV, Higher infection rates, Uygurpatients below the Han.(2)Xinjiang EBV-positive DLBCL pathogenesis common to the elderly, the peakage of onset between70~years old Han higher Uygur.(3) Xinjiang DLBCL and EBV positive DLBCL aremore common in male.(4) EBV-positive DLBCL in ethnic of age and IPI are statistically different. IPI andLDH were statistically significant between the elderly EBV-positive DLBCL and EBV-negative group, used as prognostic factors.(5)It reduced the false negative rates in detecting Epstein-Barr virus byImmunohistochemistry and In situ hybridization;(6)DLBCL might be related to EBV latent infection andbe considered as potential markers to identify large and medium-large cells in diagnosis; p100/p52proteinpositively correlated with LMP1and high expression in EBV-positive DLBCL. |
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