Analysis Of The Differentially Expression Long Noncoding RNA Profiles In Pancreatic Ductal Adenocarcinoma

ObjectiveThis study was designed to investigate the changes in long noncoding RNA(lncRNA) expression profiles between pancreatic ductal adenocarcinoma tissue andadjacent tissue, build different lncRNA/mRNA co-expression network in pancreaticductal adenocarcinoma, screen the lncRNA differentially expressed of pancreatic ductaladenocarcinoma and carry on the preliminary analysis.MethodsTotal RNA was extracted from each pancreatic ductal adenocarcinoma tissue andadjacent tissue. Integrity and concentration of RNA were assessed after RNA extraction.Then, each sample was amplified and transcribed into fluorescent cRNA. The labeledcRNA were hybridized onto the Human LncRNA Array v2.0and were scanned by theAgilent Scanner. Agilent Feature Extraction software was used to analyze acquired arrayimages. Quantile normalization and subsequent data processing were performed usingthe GeneSpring GX v11.5.1software package. After quantile normalization of the rawdata, differentially expressed lncRNA and mRNA with statistical significance betweenthe two groups were identified and establish the lncRNA/mRNA co-expression network.LncRNA may play an important role in pancreatic ductal adenocarcinoma were screenedby strategies such as change fold analysis, network neighboring genes analysis. Verify itsexpression in pancreatic ductal adenocarcinoma. The possible mechanisms ofdifferentially expressed lncRNA in pancreatic ductal adenocarcinoma were preliminarydiscussed.Results1. Compared with adjacent tissue, the lncRNA/mRNA expression profile inpancreatic ductal adenocarcinoma showed statistical significance (p<0.05). We screenedout4634differentially expressed lncRNA disordered for more than2times, including2365upregulated, and2269downregulated;612differentially expressed lncRNAdisordered for more than5times, including477upregulated, and135downregulated;4862differentially expressed mRNA disordered for more than2times, including3857 upregulated, and1005downregulated;976differentially expressed mRNA disordered formore than5times, including885upregulated, and91downregulated. DifferentlncRNA/mRNA co-expression network in pancreatic ductal adenocarcinoma wasbuilded.2. Bioinformatics analysis shows that lncRNA AK123493may play an impotant rolein the development of pancreatic ductal adenocarcinoma. The expression of lncRNAAK123493in pancreatic ductal adenocarcinoma significantly higher than the amount inadjacent tissue was confirmed by RT-PCR(P<0.05). Literature analysis suggests lncRNAAK123493may take part in the development process of pancreatic cancer through one ormore ways.Conclusion1. Compared with adjacent tissue, lncRNA in pancreatic ductal adenocarcinomatissue show differential expression.2. LncRNA AK123493may take part in the development process of pancreaticcancer through one or more ways.