Effect Of Common Edible Oils On Lipid Metabolism And Its Mechanism In Obese Mice Induced By A High Fat Diet

Objective1. To investigate the effects of several common edible oils on lipid metabolism andtheir mechanism in obese mice.2. To investigate the effects of MCT, C8:0&C10:0(3:1) and LCT on lipid metabolismand their different effects on blood lipid profiles in obese mice.3. To investigate the effects of C8:0, C10:0, C18:1and C18:0on lipid metabolism andtheir mechanism in obese mice.Methods1.15/200C57BL/6J mice were fed the basal diet as a non-obesity control. The otherswere fed with high fat diets. After4weeks, only those whose body weight gain wasover10%more than that of non-obesity control mice were chosen for the experiment.2.75obese mice were divided into5groups, which were soybean oil, MCT, peanut oil,olive oil and tea oil groups. After16weeks, body weight, body fat, diet intake, bloodlipid profiles and enzymes relevant to lipid metabolism were observed.3.45obese mice were divided into3groups, which were MCT, C8:0&C10:0(3:1) andLCT groups. After16weeks, body weight and blood lipid profiles were observed.4.60obese mice were divided into4groups, which were C8:0, C10:0, C18:1andC18:0groups. After16weeks, body weight, body fat, blood lipid profiles andtranscription factors were observed.Results1. Body weight and body fat were significantly lower in MCT, peanut oil, olive oil, teaoil groups than in soybean oi1group. The mice in MCT group showed significantlylower serum TG, TC, LDL-C, lower liver TG, TC and higher WAT cAMP, PKA, HSL,ATGL than those in soybean oil group. There was no fatty infiltration in livers of mice fed MCT, olive oil and tea oil groups, but were visible fatty liver in the others.2. The body weight, body fat were significantly lower in MCT and C8:0&C10:0(3:1)groups than those in LCT group. The mice in MCT group showed significantly lowerLDL-C in serum and higher HDL-C, HDL-C/LDL-C than those in LCT group. SerumTC and LDL-C in C8:0&C10:0(3:1) group were significantly lower than those in LCT.3. Body weight were significantly lower in C8:0, C10:0and C18:1groups than those inC18:0group. Compared to C18:0group, C8:0group had significantly lower serum TG,TC and higher HDL-C; C8:0group had significantly lower serum TG. However, C8:0group had higher LPL and lower leptin than the others. The mRNA expression ofSREBP-1and C/EBP-α in WAT in C8:0group was significantly lower than those inC18:0group. C10:0group had lower mRNA expression of SREBP-1and higherPPAR-γ in WAT than those in C18:0group.Conclusion1. Compared to soybean oil, MCT, peanut oil, olive oil and tea oil could reduce bodyweight and body fat in obese mice, MCT also decreased serum TG, TC, LDL-C andpromote lipid mobilization in WAT. Olive oil and tea oil were less obvious than MCTin blood lipids.2. Compared to LCT, MCT and C8:0&C10:0(3:1) could reduce body weight and bodyfat in obese mice with no different between them. C8:0&C10:0(3:1) also decreasedserum TC, LDL-C and lower TC than MCT.3. Compared to C18:0, C8:0, C10:0and C18:1could reduce body weight and body fatin obese mice. Βoth C8:0and C10:0could lower serum TG and induced WATtranscription factors. C8:0could reduce serum TC and HDL-C, increase WAT LPL andlower leptin. Both C8:0and C10:0did not cause visible fatty filtration in livers.In conclusion, compared to other oils, MCT could reduce body weight, body fat inobese mice and promote lipid mobilization in WAT. The MCFAs(C8:0, C10:0) couldimprove lipid metabolism with no difference.