Extraction And Biotransformation Of Xanthoceras Sorbifolia Saponin

Xanthoceras sorbifolia is a Chinese particular folk medicinal and oil plant. However, the husks of xanthoceras sorbifolia have been abandoned and have not yet been developed and utilized until now. The study on the extraction, isolation, identification, purification, enzymatic hydrolysis of xanthoceras sorbifolia saponin was very important to further knowledge and understanding of xanthoceras sorbifolia saponin. In this paper, extraction and enzyme hydrolysis of saponins from the husks of xanthoceras sorbifolia were studied to get xanthoceras sorbifolia saponins with lower sugar chain and high activity. The research on the biological activities of xanthoceras sorbifolia saponins and full utilization of xanthoceras sorbifolia resources is of great significance.At first, the extraction of saponin from xanthoceras sorbifolia was studied. The extracting conditions is that70%concentration of ethanol at reflux extraction in70℃water for5hours, and purification of xanthoceras sorbifolia saponins was finished by column chromatography. Vanillin-perchloric acid method was used for identification of xanthoceras sorbifolia saponins.In the process of enzymatic hydrolysis on xanthoceras sorbifolia saponins, the enzyme used to hydrolyze xanthoceras sorbifolia saponins was extracted. In order to screen the useful starin,2strains of Aspergillus niger sp.48s and sp.848s was used to the experiment. In the result, Aspergillus niger sp.48s was selected. After cultured, the enzyme was extracted, and the enzymatic properties were studied. The optimal pH and temperature was5.0and45℃respectively. The stable range for pH and temperature was pH3.0～pH7.0and40℃～60℃.The enzyme extracted from sp.48s was used to hydrolyze xanthoceras sorbifolia saponin. The optimal condition of enzyme hydrolysis was that concentration of substrate was3%, the pH was5.0, the temperature was50℃and the reaction time was32hours. Xanthoceras sorbifolia saponin before and after hydrolyzed was analyzed by Thin-Layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC). The results of the test show that new saponins were obtained. The structure of the new saponins needs to be analyzed in the future.