Study Of The Yangxueyufeng Granules Treatment On Vulvae Dystrophy Safety Experiment And Microcirculation

Experimental Objective:Study the effect of yangxueyufeng granules on acute toxicity and microcirculation experiments, objective evaluation yangxueyufeng granules security and the effect on improve microcirculation., for the new drugs of yangxueyufeng granules research and development provide experimental basis and clinical application.Experimental Methods:(1).The acute toxicity test:According to the results of preliminary experiments, using kunming mice to study the maximum dose experiments.(2). The microcirculation experiments:study the microcirculation of normal mouse experiments and using adrenaline caused of blood stasis mouse models microcirculation experiments, Observation yangxueyufeng granules the effect on improve microcirculation.Experimental result:(1).The acute toxicity test results:using yangxueyufeng granules240g crude drug/kg body weight administered to mice, equivalent to160times the clinical adult daily oral dosage, no mice died, Observe the heart, liver, spleen, lung, kidney till organ size, color, texture, etc. No abnormalities。Mouse body weight and organ index compared to control groups showed no significant difference.(2). The microcirculation experiments of normal mice:40minutes after the last administration, yangxueyufeng granules high, medium and low dose group can make normal mouse ear fine arteriovenous diameter enlargement、amount of open capillaries, capillary blood flow velocity, and compared to positive control group1, positive control group2and control group differences were statistically significant.(3). The microcirculation experiments of blood stasis model mice:40minutes、50minutes after the last dose, yangxueyufeng granules high, medium and low dose group can make blood stasis model mice ear fine arteriovenous diameter enlargement、amount of open capillaries, capillary blood flow velocity, and compared to positive control group1, positive control group2and control group differences were statistically significant. Experimental conclusion:(1). Using yangxueyufeng granules240g crude drug/kg body weight administered to mice, equivalent to160times the clinical adult daily oral dosage, no mice died.Mouse body weight and organ index compared to control groups showed no significant difference, so we can see that the yangxueyufeng granules by taking courses intended clinical dose and is safe and reliable.(2) Yangxueyufeng granules can improve the normal mouse that40minutes after the last dose ear fine vein diameter, the amount of open capillaries, capillary blood flow velocity,and improve40minutes、50minutes after the last administration blood stasis model mice ear fine vein diameter, the amount of open capillaries, capillary blood flow velocity, shows that yangxueyufeng granules has a certain effect on improve microcirculation.