Study On Components From The Twigs Of Trigonostemon Xyphophylloides

Trigonostemon xyphophylloides belongs to the genus Trigonostemon (Euphorbiaceae), which is composed of about50species mostly distributed in tropical and subtropical regions of Asia. There are10species in China, which are mainly distributed in provinces of south China. Plants of the genus Trigonostemon are used as traditional medicine to cure asthma, poisonous snake bites and food poisoning in Thai folk medicine. Phytochemical studies on plants of this genus have lead to the isolation of many kinds of compounds, including diterpenoids, alkaloids, flavonoids, coumarins, lignans, and steroids, which showed a variety of biological activities, such as insecticidity, antimicrobial, anti-HIV-1activity, cytotoxicity and so on. Trigonostemon xyphophylloides is a shrub endemic in Hainan province. Previous research of the plant has led to the isolation of daphnane diterpenoids, which exhibited strong cytotoxic activity. To search for new and cytotoxicity compounds from T. xyphophylloides, the ethanol extract of its twigs were studied for the purpose of developing this plant resource.In this study, twenty seven compounds were isolated by all kinds of modern separation technology. On the basis of spectroscopic data (UV, IR, MS,1D and2D NMR) and physicochemical properties, their structures were identified as trigoxyphin J (1), trigoxyphin K (2),3-O-benzoylpluricostatic acid (3), trigoxyphin A (4), trigohownin D (5), trigoxyphin D (6), trigohownin B (7), trigohownin C (8), trigohownin F (9), trigoheterin B (10), trigochinin E (11), neoboutomannin (12),1,2-dihydroheudelotinol (13), pluricostatic acid (14),24(R),25-dihydroxy-5a-dammar-20-en-3-one (15), ursolic acid (16), Δ8,22-ergostadien-3β-ol-11-one (17), mangdesisterol (18), stigmastane-3β,6a-diol (19), ergosterol peroxide (20),β-sitosterol (21), daucosterol (22), jacarreubin (23),6-deoxyjacareubin (24), syringaresinol (25), episyringaresinol (26),1-palmitic acid ester (27). Compounds1and2were new daphnane diterpenoids,3was a new friedelane triterpene derivative, and compounds5and7-27were first obtained from the genus Trigonostemon.All of the compounds were evaluated for cytotoxic activities against K562, SGC-7901, and BEL-7402cell lines, using MTT method. The results showed that1and2exhibited strong activity against K562with IC50values of8.3and7.8μg/mL, respectively. The antimicrobial activities of all the compounds were also assayed using paper diffusion method and the results indicated that compounds12,17,18and26showed effects against Staphylococcus aureus.