Changes Of MiR-21and MiR-210after Hydrogen-rich Saline Treated Cerebral Ischemia-reperfusion In Hippocampus Of Rats

Objective The ischemia-reperfusion model was set up with Pulsinelli’s4-vessel occlusion to investigate the changes of miR-21and miR-210expressions in impaired hippocampus after hydrogen-rich saline treated cerebral ischemia-reperfusion injury in rats, thereby it can establish a brand new way of precaution, prognosis and therapy for cerebral resuscitation.Methods Seventy-two male SD rats, aged9-10weeks, weighing250-300g, were randomly divided into3groups(n=24):sham operation group(group Sham), ischemia reperfusion group(group IR), hydrogen-rich saline group(group H). Global cerebral ischemia-reperfusion was induced by four-vessel occlusion.In group IR and H, global cerebral ischemia reperfusion was induced by15min ligation of bilateral carotid artery followed by24h or72h reperfusion. In group H intraperitoneal0.6mmol/L hydrogen-rich saline5ml/kg was injected immediately after reperfusion and after6h reperfusion, while the equal volume of normal saline was injected instead of hydrogen-rich saline in the other two groups. Six rats of each group were sacrificed after24h or72h of reperfusion, and then the hippocampus was removed for detecting the expressions of miR-21and miR-210by qRT-PCR. Then another6rats were sacrificed at the end of72h or24h reperfusion, the pathological changes of pyramidal neurons and the number of normal pyramidal neurons were evaluated in hippocampal CA1region by using HE staining.Results①Compared with group sham, the miR-21expression in group IR were increased significantly(.P=0.00); Compared with group I/R, the expression in group H was decreased significantly (P=0.00).②Compared with group sham, the miR-210expression in group I/R was increased significantly(P=0.00); Compared with group I/R, the expression in group H was decreased significantly (P=0.00).③ Compared with group Sham, the number of pyramidal cells at24h or72h of reperfusion in CA1region of hippocampus in group I/R and H were decreased significantly (P=0.00each); Compared with group I/R at24h, the number in group I/R at72h was decreased significantly (P=0.03), and the number in group H at24h was increased significantly (P=0.00); Compared with group I/R at72h, the number in group H at72h was increased significantly (P=0.00).④The expression of miR-21and miR-210were negative correlated with the number of pyramidal cells in each groups(P=0.00).Conclusions The expressions of miR-21and miR-210decreased significantly in hydrogen-rich saline treated rats, the dynamic changes of miR-21and miR-210may indicate the severity of ischemia reperfusion injury and the therapeutic efficacy of hydrogen-rich saline. MiR-21and miR-210may play a role in the mechanism of hydrogen-rich saline attenuated the cerebral ischemia reperfusion.