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The Study On The Influences Of Gastrointestine And Behavioristics After Blocking Low Hydraulic Resistance Channel Along The Stomach Meridian In Mini-pigs

Posted on:2015-05-19Degree:MasterType:Thesis
Country:ChinaCandidate:W T ZhouFull Text:PDF
GTID:2284330467488931Subject:Integrative basis
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The essence of the Traditional Chinese Medicine is the pursuit of the union between man and nature, man and society and among every parts of body. Meridians connect between the body surfaces, body surfaces and organs (Zang-fu) and integrate every parts of the body. It is one of the most important systems which reflect the integral characteristics of the body. Meridians are closely related to the physiological function, pathological process and the mechanism of acupuncture treatment.Meridians and collaterals are the core in TCM theory. The correlation between meridians and organs (Zang-fu) is an important aspect of meridian theory. The objective of this paper is to further investigate the function of the internal organs and the relationships between the internal organs and meridians. In order to reach this objective, after reviewing the previous research the author continues building the.mini-pigs’pathological model of "meridian impassability" through blocking the stomach meridian by injecting gel into the channel and increases the number of samples. Firstly, the author observes the change of the states of mini-pig’s stomach and intestine. Secondly, the author observes the several behavioral indicators of the pathological model. Monitoring these behavioral indicators lays the foundation for the study of TCM pathological model and the therapy of disease by Chinese medicine.Experiment1The study of the effects on mini-pigs’stomach and intestine by blocking low hydraulic resistance channel (LHRC) along the stomach meridian.ObjectiveThe objective of this experiment is to investigate the pathological changes in the organs resulting from blocking low hydraulic resistance channel (LHRC) along the stomach meridian by injecting gel in pigs so as to offer some insight into the correlation between meridians and internal organs. Method16same strain black mini-pigs were divided into four batches and were raised in the cage in deferent periods. Each batch included four pigs and the pigs with similar conditions were made pairs. The pigs in experimental group which was injected0.5-1.0ml of gel into6to8low hydraulic resistance points along the stomach meridian, and build the pathological model of "meridian impassability". The same volume of saline was injected into the same points of the pigs of the first batch, second batch, and the third batch in the control group. The fourth batch of the control group was injected0.5-1.0ml of gel into6low hydraulic resistance points along the kidney meridian. So the statistics was not included the data of the fourth batch. After be raised in cage6to10weeks they were dissected and observed the state of stomach and intestine and the body gel distribution state.ResultsThe results showed that all of the eight pigs which were blocked LHRC have different degrees of flatulence or/and, tympany in the he stomach and intestine. While there were no abnormal states in six control pigs, except that one pig died of inappropriate anesthesia during the experiment. The findings confirmed that the blockage of LHRC along the stomach meridian can result in the abnormal state of stomach and intestine.ConclusionThe findings confirmed that the blockage of LHRC along the stomach meridian can influence the state of stomach and intestine, leading to the distension on stomach and intestine. It conformed the description of the abdominal distension in the symptoms of the stomach meridians.Experiment2The study of the effects on mini-pigs’behavioristics by blocking low hydraulic resistance channel (LHRC) along the stomach meridian.Objective The objective of the experiment is to study the behavioristics of the pathological model by blocking low hydraulic resistance channel (LHRC) along the stomach meridian by injecting gel in pigs and finds out whether mini-pigs’behavioristics would change after the blockage of meridians. If the behavioristics change, in what way will it change?MethodExperiment2and Experiment1were conducted at the same time. On the base of the mini-pigs’“blocking model", those mini-pigs were observed6to10weeks. The author used the multi-route video surveillance systems of24hours to conduct a real monitor of each pig. At night the systems automatically converted into the mode of infrared camera and recorded the behaviors of mini-pigs. The author recalled the video after saving the video, replayed it at different speeds, and conducted the analysis of behavioristics. The content of the monitoring is made up of four behavioristic indexes; which were the times of nighttime movement, the time of the nighttime movement, the times of climbing bars in the daytime, and the time of lying in the daytime.ResultBehavioristic index of experimental group including the times of nighttime movement, the time of the nighttime movement, the times of climbing bars in the daytime, all increased after the blockage of LHRC. Especially a significant association was found between the times of climbing bars in the daytime and the modeling time. That correlation coefficient is as high as0.868, which had the statistical significance (P<0.05). The times of climbing bars in the daytime in the control group hasn’t this kind change, the correlation coefficient is-0.045(P>0.05. Among all the N-of-ones, the times of nighttime movement of the NO.1experimental pig increased more than before the modeling, which had the statistical significance (P<0.05).However, there were no significant differences between before and after modeling in the control pig in the same group. The increased times of nighttime movement and climbing bars had similarities with the description of the "abnormal function in sleep and stomach" and "climbing high to sing" in the symptoms of the stomach meridians. All those showed that blocking LHRC after some time can cause certain the symptoms of the stomach meridians, and the behaviors were more and more obvious with the increase of the time of the "blocking model". Which echoed the phenomenon of stomach’s bloating observed during the dissection.ConclusionThe blockage of the meridian can result in a lot of behavioristic change which relates to the symptoms of the stomach meridians.As can be seen from results of the two experiments:The blockage of meridians can cause the pathological change of related internal organs and the change of the behaviors. If people don’t care about it and tone, the related pathology of function and organ will show over time. The sub-healthy state converts into the illness. All the results show that cyclic low hydraulic resistance channel (LHRC) is one of the most important essences of the TCM meridians from the perspective of the correlation between meridians and internal organs. At the same time, they show that the method which produces the pathological model of blocking the meridians by injecting gel is feasible.
Keywords/Search Tags:Meridians, Low Hydraulic Resistance Channels, Pathological model, Behavioristics, Mini-pig, Gel
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