Mountain Cornus And Its Extract Mono Glycosides Research In RIR Related Injuries

Objective:Retinal ischemia reperfusion (RIR) damage is the eye of the pathological process of clinical disease, mainly in ischemic retinal diseases and glaucoma patients, and ischemic ophthalmopathy.Restored blood supply in the retina of apparent dysfunction decreases vision, damage optic nerve and defect visual field such as clinical symptoms, and even irreversible damage occurs greatly undermined the physiological function of the retina. Current research on retina ischemia-reperfusion injury, reperfusion does not alleviate the damage of retinal cell, exacerbate the cell death. In terms of RIRs damage mechanism, current research focus on that which excites theory of amino acid, calcium channel theory, theory of cell apoptosis, inflammation doctrine theory, theory of oxygen free radicals and nitric oxide, apoptosis gene theory, etc. In its mechanism, the occurrence of retinal ischemia-reperfusion injury is not a single factor, the multi-drug treatment will begin. Explore and try to use many different drugs for treatment in accordance with rirs damage mechanism theory. Likelihood of traditional Chinese medicine in the treatment of RIRs damage related to clinical disease research is still in the exploratory stage, there is plenty of room is worth mining. In recent years, a large number of experimental data demonstrate that Cornus officinalis extract mono glycosides to inhibit the apoptosis of nerve cells have a protective effect. By inference, in the treatment of retinal ischemia-reperfusion injury caused by anterior ischemic optic neuropathy of cornus to join in the Cornus officinalis, the formulas can improve the clinical curative effect, further verify the mono glycosides of retinal ganglion cells protection. Purpose:To summary of traditional Chinese medicine (TCM) Cornus officinalis, medication regularly by retrospective investigation and analysis of clinical using of Cornus officinalis in anterior ischemic regard mental derangement,To provide theoretical and experimental basis by the establishment of the thought and method through the experiment, the preliminary exploration Cornus officinalis extract mono glycosides of hypoxia in sugar state under the influence of retinal ganglion cell activity, for the mono glycosides related preparation drugs in the clinical application. Methods:Retrospective study:the choice is between November2010to December2013cases in the treatment of anterior ischemic optic neuropathy in induction to summary of the system. According to the conditions of drug utilization of the original case records and combine, in the process of diagnosis and treatment of patients with visual acuity and visual analysis using the Cornus officinalis, to justificate of cornus usage time and the use of summary Cornus officinalisclinical thinking and role in the treatment of the disease. Using SPSS18.0statistical data and processing. Measurement data with x+s, using the single factor analysis of variance (ANOVA), two comparison by using LSD-t test. Laboratory methods:Using immunofluorescence method appraisal RCG-5cells, at the same time under the low oxygen sugar retinal ganglion cells RGC-5training, and then placed in different concentrations of mono culture medium (low dose (including10mol/L,100(including high dose mol/L) and control group in the medium, determined by MTT method to detect cellular activity.The results:Statistical results for clinical retrospective case found that the anterior ischemic neurology clinic to the total number of cases of232cases, application of treatment in prescriptions containing Cornus officinalis number of cases, a total of37cases, application cases containing Cornus officinalis accounts for about15.95%of the total number of cases. In cases of cornus applications including Cornus officinalis, in the liver and kidney Yin deficiency and blood stasis syndrome "and" qi and Yin deficiency Fried mesh winding stasis syndrome "cases is in the majority of patients, followed by:" qi deficiency and blood stasis syndrome ","qi deficiency blood stasis", "liver depression and qi stagnation syndrome".Identified by immunofluorescence RCG-5:cell cytoplasm to tan, the nucleus to violet.The influence of mono glycosides of retinal ganglion cells MTT experiment①The condition of low oxygen sugar can cause retinal ganglion cell activity;②low oxygen sugar group compared with normal cells, its each time OD value significantly reduced (P<0.05);③mono nucleoside group10mu g/ml role24h its OD value low sugar low oxygen control group increased significantly (P<0.05);④ith the increase of concentration of mono nucleoside, mono glycosides high concentration group100g/ml each time OD values were significantly increased in the hypoxia group, the OD value of cell proliferation activity of the highest in the hypoxia group;⑤ono glycosides can improve the low oxygen in sugar caused by the role of the ganglion cell activity, has certain promoting effect on cell proliferation, and the dependence on dose.Conclusion:Cornus officinalis prescriptions for most of the treatment of anterior ischemic neuropathy is in the position of "zuo"and "shi".Mono glycosides can improve the low oxygen in sugar caused by the role of the ganglion cell activity, has certain promoting effect on cell proliferation, and the dependence on dose.