The Protective Effect Of Zhiiongtongluo Tablets On Cerebral Ischemia Reperfusion Injury In Rats

Objective: Cerebrovascular diseases have the characteristics of high morbility, high rate ofmutilation and high mortality; therefore they can do great harm to human health. The presentstudy was performed to test the protective effect of Zhilongtongluo Tablets on focal cerebralischemia-reperfusion in rats and explore its potential mechanisms.Methods: Wistar male rats (120) were randomly divided into the sham group, theischemia-reperfusion group, the ZLTLT treated group (0.405,0.81,1.62g/kg) and theNaoxintong Jiaonang treated group (2.16g/kg). The different doses of ZLTLT wereadministrated to rats by lavage for7days. Rats were subjected to24h of reperfusion followingischemia for2h induced by middle cerebral artery occlusion (MCAO). The neurologicalbehavioral test was performed24h after reperfusion. The changes of brain water content,cerebral index, the activity of SOD and the content of MDA in brain tissue were measured.Results:①Compared with sham group, the neurological deficit was increased in modelgroup after24h reperfusion (P<0.001). Pretreatment ZLTLT could decrease the neurologicaldeficit compared with model group (P<0.05, P<0.01).②Compared with sham group, thebrain water content and cerebral index were increased in model group after24h reperfusion(P<0.001). Pretreatment ZLTLT could decrease the brain water content and cerebral indexcompared with model group (P<0.05, P<0.01).③Compared with sham group, the bloodviscosity and plasma viscosity increased in model group after24h reperfusion (P<0.001).Pretreatment ZLTLT could decrease the blood viscosity and plasma viscosity compared withmodel group (P<0.05, P<0.01).④Compared with sham group the activity of antioxidantenzyme superoxide dismutase (SOD) was significantly decreased, while the content ofmalondiadehyde (MDA) was increased significantly in the model group after24h reperfusion(P<0.001). Pretreatment ZLTLT could increase the activity of SOD and decrease the content ofMDA compared with model group (P<0.05, P<0.01).⑤Compared with sham group thecontent of TXA2content was significantly higher (P<0.01or P<0.001), while the content ofPGI2and PGI2/TXA2ratio were lower (P<0.01or P<0.001) in the model group after24hreperfusion (P<0.001). Pretreatment ZLTLT could increase the content of PGI2and decreasethe content of TXA2compared with model group (P<0.05, P<0.01).⑥Compared with shamgroups, most of the neurons in cortex and hippocampal disappeared in the model groups. Pretreatment ZLTLT could significantly attenuate the pathophysiological changes ofhippocampal neuronal cells.Conclusion: These results demonstrated that ZLTLT significantly protected cerebral ischemiareperfusion injury in rats by antioxidant.