The Imbalance Between Th17and Cd4~+Cd25~+Regulatory T Cells In Patients With Hashimoto’s Thyroiditis

Objective:Through observing the expression of related cytokines of Thl7and CD4+CD25+regulatory T cells between thyroid and peripheral blood in Hashimoto’s thyroiditis patients to explore the role of Th17/Treg balance in the pathogenesis of HT.Methods:(1) Thyroid and peripheral blood were collected from10patients with HT who developed hypothyroidism,10patients with HT who were euthyroid and10control subjects without HT.The expression of IL-17A and Foxp3mRNA in thyroid were measured by RT-PCR.(2) The levels of related cytokines IL-17,IL-10were assayed by ELISA methods.(3) Electrochemiluminescence immunoassay (ECLI) was applied to measuring the contents of FT3,FT4,TSH.Results:(1) The expression of IL-17A and Foxp3mRNA in thyroid tissues.①In patients with HT, the expression of IL-17A mRNA in thyroid increased significantly,which of hypothyroidism group were higher than euthyroid group (P<0.05)②In patients with HT, the expression of Foxp3mRNA in thyroid decreased significantly in HT groups,but there were no significant difference between hypoth-yroidism group and euthyroid group.(2) The levels of associated cytokines IL-17,IL-10in serum.①In patients with HT, the serum IL-17levels increased significantly,which of hypothyroidism group were higher than euthyroid group (P<0.05)②The serum IL-10levels decreased significantly in HT groups,but there were no significant difference between hypothyroidism group and euthyroid group infection group than that in normal control. Conclusion:The increased response of Th17cells and immunological function deficiency of Treg cells are presented between thyroid and peripheral blood in HT,suggesting that the imbalance between Th17and Treg may participate in the process of HT.