Active Ingregients Of Shandong Famous Region Drug Extraction Isolation And Identification

Objctive: Study the extraction, separation,purification method and characterizationtechnique of the active ingredients in Flos lonicerae, Radix Salviae Miltiorrhizae, RadixScutellariae Baicalensis,Fructus et Semen Trichosanthis Kirilowii,Radix seu HerbaCynanchi Paniculati and Ginkgo biloba L. They are famous-region drug of ShandongProvince.To establish separation parameters which separation and throughputmaximization required and determine the pervasive optimizing preparation technology foractive constituents above.Methods: To extract the active ingredients of famous region drug by alcoholrefluxing extraction and steam distillation methods.To separate and purifying activesubstance by two-phase solvent extraction, rapid liquid phase separation, adsorption ofmacroporous resin column chromatography, high-speed countercurrent chromatography,polyamide column chromatography, ODS chromatography methods.To quantitativeanalyse the active ingredients by HPLC methods.To dentificate the structure of activeingredients by thin layer chromatography, infrared spectroscopy, ultraviolet spectrometry,mass spectrometry, nuclear magnetic resonance methods.Results: The preparative technique of active substance from Flos lonicerae, RadixSalviae Miltiorrhizae, Radix Scutellariae Baicalensis, Fructus et Semen TrichosanthisKirilowii, Radix seu Herba Cynanchi Paniculati and Ginkgo biloba L. can realize sampleof fast and efficient separation and purification, realize preparation of standard substanceof active substance on the gram level. To determinate melting of active substance,establishinfrared spectroscopy,ultraviolet spectrometry, mass spectrometry, nuclear magneticresonance library, realize the rapid identification of the related compounds.