Based On Enzyme Circulation Amplification Of Surface-enhanced Raman Spectroscopy Technology In The Application Of Tumor Markers Detection Research

In this study, several ultrasensitive and high selectivity surface enhanced Ramanscattering (SERS) methods has been designed to selectively and sensitively detect theDNA, lysozyme, and Ramos cells. The aptamer-based target-triggering cascademultiple cycle amplification consists of strand displacement amplification and nickingenzyme signaling amplification, leading to significant SERS enhancement. Thosemethods provided basic research data for the detection of other biological molecules.This paper research content mainly includes the following aspects:1. This experimental study a new method to detect the DNA, through nickingenzyme signaling amplification and strand displacement amplification. With theamplification process and the Raman probe and the gold film, we got a significantSERS enhancement, implements the high sensitive and selective detection of DNA.The method through the target DNA caused enzyme replacement cycle amplificationand chain reaction, produce a large number of Raman probe on the gold base,significantly enhanced Raman signal. In addition, the experiment using magnetic bead,effectively reduce the background signal. This detection method exhibits excellentspecificity and sensitivity towards DNA with a detection limit of2.7×10-14M. Thismultiple amplification SERS strategy has many advantages: simple, high sensitivity, selectivity, wide applicable, and through a simple change of the DNA sequence thatcan be used for other metabolites, protein, and the detection of biological cofactors.2. This experiment based on aptamer interact with its target protein specificityrecognition, combining with the enzyme circulation amplification technology, putforward a new method for detection of lysozyme with used the targeted activationsource amplificate SERS signals. The aptamer-based target-triggering lysozymeaggregate and replacement cycle amplification and nicking enzyme signalingamplification, to get a lot of magnetic beads-gold nanoparticles (AuNPs) bio-barcodeRaman probe which cause lysozyme content related Raman signals. This ultrasensitivesurface enhanced Raman scattering (SERS) method has been designed to selectivelyand sensitively detect the lysozyme, the limit of detection is8.3×10-15M. This methodprovides a convenient, rapid, high sensitive detection for lysozyme, and the methodalso has wide applicability and has broad application prospects in other protein andtumor markers detection areas.3. This system constructed a new type of method used SERS and based nickingenzyme signaling amplification to detect Romas cell. With the DNA aptamer to cellsurface protein has specificity and high combined strength, nicking enzyme signalingamplification, and the gold nanoparticles (AuNPs) bio-barcode Raman probeenhancement on the gold substrate are employed to enhance the SERS signals.Achieved high sensitivity and high selectivity for the detection of target DNA orlymphoma Ramos cells. The method of the detection of target DNA and Ramoslymphatic cell got the detection limit is2.1×10-15M and87cells. This test methodin the field of tumor early diagnosis and genetic testing has a broad applicationprospect.