| Objective: As the most powerful antigen presented cells, dendriticcells (DC) are the activator of body’s immune response, which stimulate theoriginal T cells and induce immune response. DC can express receptor which inantigen uptake and transportation of special membrane. It can effectivelyabsorb and deal with the antigens, and migrate to T cells to induce Tlymphocytes into mature ones. DC becomes mature after migrating. Thenexpress a large number of molecular MHCⅡ class, providing specific signal toCD+4T cells. Studies have proved that DC could induce the CD+4T cellsdifferent into T helper lymphocyte (Th). The balance of Thl/Th2cells is veryimportant to the body’s normal immune function. It is widely recognized thatthe immune status of nasal polyp tissues is abnormal. There is lymphoid tissueinfiltration, and imbalance of Thl/Th2cells. In this study, the expression levelsof DC label CD83, Tumor necrosis factor-α(TNF-α), Interleukin-4(IL-4),Eosinophils(EOS) in nasal polyp tissues before and after the glucocorticoidtreatment were detected so as to explore the role of DC in the immuneregulation of nasal polyps, and the affect to DC in nasal polyps afterglucocorticoid treatment.Methods:30patients with nasal polyps before andafter glucocorticoid treatment were selected hospitalized as experimental groupin the department of Otolaryngology Head and Neck Surgery, the affiliatedhospital of Luzhou Medical College from June2013to December2013. Allpatients in the experimental group took systematic and local glucocorticoid treatment for4~7days. At the same time,18turbinate mucosas were selectedfrom patients underwent nasal septum deviation diorthosis as control group.Immunohistochemical technique (SABC method) and in situ hybridizationtechnique were used to detect the expression of CD83, TNF-α, IL-4, and HEstaining was use to detect the expression of EOS. Image-Pro Plus Imageanalysis software were used to get the average optical density value of CD83,TNF-α and IL-4positive cells under a microscope. The numbers of EOS cellswere also counted under a microscope. SPSS17.0software was used to analyzethese data. Results:1. Expression of CD83positive cells. The mesn opticaldensities(MOD)were0.1618±0.0578and0.2048±0.0380respectively forimmunohistochemical and in situ hybridization staining before glucocorticoidtreatment,and0.0359±0.0149and0.0505±0.0189after glucocorticoidtreatment. and0.0075±0.0027and0.0059±0.0013in the control group.Compared the control group to before and after glucocorticoid treatment, thedifferences were statistically significant; all P<0.05. Compared beforeglucocorticoid treatment to after glucocorticoid treatment, the differences werestatistically significant, all P<0.05.2. Expression of TNF-α positive cells.Expression of CD83positive cells. The mesn optical densities(MOD)were0.152±0.0557and0.0668±0.0201respectively for immunohistochemical andin situ hybridization staining before glucocorticoid treatment,and0.0576±0.0232and0.0388±0.0134after glucocorticoid treatment. and0.0061±0.0022and0.0075±0.0032in the control group. Compared the control group to before and after glucocorticoid treatment, the differences werestatistically significant, all P<0.05; Compared before glucocorticoid treatmentto after glucocorticoid treatment, the differences were statistically significant,all P<0.05.3. Expression of IL-4positive cells. Expression of CD83positivecells.The mesn optical densities (MOD) were0.2314±0.0434,0.1419±0.0552respectively for immunohistochemical and in situ hybridization staining beforeglucocorticoid treatment,and0.0228±0.0059and0.0341±0.0148afterglucocorticoid treatment.and0.0031±0.0014and0.0056±0.0024in the controlgroup.Compared the control group to before and after glucocorticoid treatment,the differences were statistically significant, all P<0.05; Compared beforeglucocorticoid treatment to after glucocorticoid treatment, the differences werestatistically significant, all P<0.05.4. Expression of EOS. The number beforeglucocorticoid treatment was45.24±14.80, and the number after glucocorticoidtreatment was14.99±5.72; the number of control group is4.08±1.49. Comparedthe control group to before and after glucocorticoid treatment, the differenceswere statistically significant, all P<0.05; Compared before glucocorticoidtreatment to after glucocorticoid treatment, the difference was statisticallysignificant, P<0.05.5. Before glucocorticoid treatment by immunohistochemicaland in situ hybridization staining, the expression levels of IL-4in the tissue ofnasal polyps were higher than TNF-α, difference was statistically significant(t=6.197,6.197,P<0.05);After glucocorticoid treatment byimmunohistochemical and in situ hybridization staining, the expression level of IL-4in nasal polyps were lower than that of TNF-α, difference was statisticallysignificant (t=7.996,7.996, P<0.05).6. The correlation analysis of theexpression of CD83and TNF-α: there was a positive relationship between theexpression of CD83and TNF-α before and after glucocorticoid treatment byimmunohistochemical and in situ hybridization staining, P<0.05.7. Thecorrelation analysis of the expression of CD83and IL-4: there was a positiverelationship between the expression of CD83and IL-4before and afterglucocorticoid treatment by immunohistochemical and in situ hybridizationstaining, P<0.05.8. The correlation analysis of the expression of CD83andEOS: there was a positive relationship between the expression of CD83andEOS before and after glucocorticoid treatment by immunohistochemical and insitu hybridization staining, P<0.05. Conclusions:1.The increased expressionof CD83which was the marker of mature DC in nasal polyp tissues showedthat the DC participated in the process of the occurrence and development ofnasal polyps.2.TNF-α and IL-4were represents factors of Th1and Th2cells.Their expression levels were significantly higher than the control group beforeglucocorticoid treatment and the expression of IL-4level was higher thanTNF-α, It suggested that Th1/Th2mixed expression in nasal polyps and DCmight induce Th0cells differentiate to Th2cells, the Th2cells predominated.3.After glucocorticoid treatment, the expression levels of TNF-α and IL-4weresignificantly lower than that before glucocorticoid treatment, but higher thanthe control group and the expression of IL-4levels was lower the TNF-α. It suggested that after glucocorticoid treatment intervention DC might inhibit theTh2differentiation, inducing Th0differentiate to Th1cells and the Th1/Th2proportional reversal in nasal polyp tissues.4.The expression of CD83and EOSwere positively correlated, which suggested that CD83(DC) and EOS helpedeach other forward in the occurrence and development of nasal polyps.5.Further studies of nasal polyps organization relationship between DC and itsrelated cytokines might improve the understanding of the pathogenesis of nasalpolyps. Further studies of the steroid glucocorticoids to DC and the cytokines innasal polyp tissues should provide new theoretical basis and target of theprevention and treatment for nasal polyps. |
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