Effect About Arsenic Trioxide Combined With Gemcitabine On Human Lung Adenocarcinoma A549Cells’ Proliferation Inhibition And Reception Of COX-2and VEGF

Objective：According to the Arsenic trioxide, Gemcitabine and two drugcombination on the human lung adenocarcinoma A549cell growth, detectthe effect about the rate of proliferation inhibition and expression ofCOX-2and VEGF, so as to may finding new medicine and possibletheoretical that can be use to improve the lung cancer therapy. Methods：Choose the logarithmic growth phase of the human lung adenocarcinomaA549cells which are cultivated in vitro as the experimental group treatedcells, cultivated by RPMI-1640medium, which contains10%FBS, in the5%CO2、37℃incubator. Time period was divided into two（48h and72h）,and each time period was divided into four groups according to the drugs:Group2use Arsenic trioxide only，the drug concentration is8μmol/L，Group3use Gemcitabine only, the drug concentration is0.01mg/L，Group4use Arsenic trioxide combine with Gemcitabine， the drugconcentration is the same as Group2and Group3，and Group1as thecontrol group. We though the MTT method to detect the rate of cells’proliferation inhibition of different groups of drugs and using ELISAmethod to detect the expression of COX-2and VEGF. Results：Comparing with the control group, Arsenic trioxide and Gemcitabine alone on the human lung adenocarcinoma A549cells can inhibit cells’proliferation, and down regularly the expression of COX-2and VEGF,the difference was statistically significant（P＜0.05）; while Arsenictrioxide combined with Gemcitabine on human lung adenocarcinomaA549cell proliferation inhibitory effect is stronger in the group withArsenic trioxide or Gemcitabine alone, and down the expression ofCOX-2and VEGF as well, the difference has statistically significance（P＜0.05）.Conclusion：Arsenic trioxide combined with Gemcitabine caninhibit in vitro human lung adenocarcinoma A549cells’ proliferation, themechanism may be connected with down-regulation of COX-2andVEGF expression.