Screening Of Anticancer Activity Of Three Kinds Of Radix Aconiti And The Index Components, And Anti-inflammatory And Analgesic Activity Of Aconitum Karakolicum

Objective:According Screening of Anticancer activity of three kinds of Radix Aconitiand the index componens，and the anti-inflammatory and analgesic activity of the differentextracts of Aconitum karakolicum，to prepare for expand the development of Xinjiang realestate monkshood medicinal material and study pharmacological and properties providingthe basic research data. Methods:(1) The Aconitum component extraction: on three kindsof Aconitum were extracted and separated by different polar solvents of different method,obtains the different polar fractions extract,calculate the rate of paste (2) Screening ofvarious fractions of aconitine in vitro antitumor activity: through the MTT method,for thedetection of different samples in vitro inhibition of tumor cellgrowth activity,data usingBliss method by SPSS software to calculate IC50value,according to the experimentaldesign of the data packet statistics.(3) Aconitum karakolicum acute toxicity experiment:ICR mice once a day by intragastric administration of extracts from different parts ofdifferent doses ofAconitum karakolicum, observation of acute toxicity in mice and deathrecords,using the method of Bliss program to calculate the median lethal dose(LD50)andits95%confidence interval.(4) Aconitum karakolicum anti-inflammatory experiment:ICRmice were administered intragastrically with different extraction fractions of Aconitumkarakolicum liquid,the last30minutes after administration,each mouse ear outside thesmearxylene30μL，comparison of Aconitum karakolicum components to inhibit earswelling rate.(5) Aconitum karakolicum analgesia experiment: ICR mice wereadministered intragastrically with different extraction fractions of Aconitum karakolicumliquid,the last45minutes after administration,mice were intraperitoneal injection of0.6%acetic acid solution0.2mL/10g, continuous observation of15minutes, to observe and record the number of writhing response within15minutes of each mouse,more root ofAconitum components on inhibition writhing reaction rate. Results:(1) Different parts ofthe extract rate comparison: water extract part of Junggar.The highest rate ofpaste,cyclohexane extract part of Aconitum karakolicum extractrate was the highest, theethyl acetate extract part of Junggar.The highest rate of paste,90%ethanol extract part ofAconitum karakolicum extract rate was the highest (2) Effect of antitumor activity ofAconitum leucostomum is the best, followed by Junggar. The effect of the indexcomponents; the best is12-epi-napelline,followed by Song Guoling and Lappaconitinehydrobromide; extract is the best of cyclohexane extracts, followed by water and90%ethanol extracts; tumor cells the effect of extract on the digestive system and reproductivesystem the best effect;tumor cell index components on the reproductive system of the best(3) Aconitum karakolicum water extract LD50dosage: adult female miceapproximatelyequivalent to the clinical dose of1.5times, Aconitum karakolicumcyclohexane extractLD50dosage: adult female mice is equivalent to about66times the dose of clinical use ofaconite, ethyl acetate extract dose: a plurality of LD50female mice approximatelyequivalent to the adult clinical dose of17times,Aconitum karakolicum90%ethanolextract of LD50dosage:adult female miceapproximately equivalent to6times the clinicaldose,(4) Aconitum karakolicum water extract group, cyclohexane extract group, ethylacetate extract group and90%ethanol extract group para xylene induced mouse ear edemainhibition rate was1.75%,28.65%,32.75%and22.81%;(5) Aconitum karakolicum waterextract group,cyclohexane extract group, ethyl acetate extract group and90%ethanolextract group due to acetic acid inhibitionrates were32.41%,28.8%,61.38%and26.91%mice writhing times. Conclusion:(1) Aconitum karakolicum extract rate was the highest.(2) Three species of the tumor cell growth inhibition has a certainrole.(3). A plurality ofblack water extract group the most toxic, cyclohexane extract groupwith minimal toxicity.(4) Aconitum karakolicum on acute inflammation of the effective part of ethyl acetateextract part.(5) The effective parts of a plurality of Aconitum analgesia for ethyl acetateextract part.