Experimental Role Of Stellate Ganglion Block At Early Stage Speed Up The Facial Nerve Injury And Regeneration Of Rats

Objective:To explore the effects of Stellate Ganglion block on facial nerve regeneration in rats and find the best time possible to treatment.Methods:A total of72adult Sprague-Dawley rats were devided into4groups of sham-operation (Sham group), model control (NS), SGB1and SGB2(n=18each) according to a random number table. Except for sham group, the model of peripheral facial paralysis was established for three other groups. After treating facial nerves injury with SGB at different timepoints, the behavioral changes of rats were observed, Electroneurography (ENoG) of injured nerves was performed and the expressions of Glial cell line-derived neurotrophic factor (GDNF) of the facial nerve nucleus were detected at different stages.Results:The facial whiskers movement function score in Sham group、NS group、SGB1 group and SGB2group were3.92±0.2,0.67±0.41,1.67±0.41and0.75±0.27(P=0.004) at the end of7d, being3.87±0.26,0.83±0.26,2.17±0.52and1.67±0.26(P=0.000) at14d, being3.92±0.2,1.73±0.29,2.92±0.2and2.58±0.38(P=0.001) at28d。7days later, the LatSD andAmpSD were not detected in NS group, SGB1group and SGB2group. But14d later, the values of LatSD were (1.34±0.24) ms、(6.64±0.20)ms、(4.64±0.47)ms and (6.43±0.45) ms, showing a significant difference (F=274.723, P=0.000), and AmpSD were (6.17±1.21)mv、(2.26±0.48) mv、(4.54±0.56) mv and (3.66±0.44)mv, also giving a significance (F=29.504,P=0.000); And28d later, LatSD were (1.34±0.12) Oms,(3.13±0.19)ms,(1.98±0.04)ms and (3.03±0.16) ms (F=235.699, P=0.000), AmpSD were (7.57±0.36) mv,(4.12±1.03) mv,(6.74±0.40) mv and (5.43±0.48) mv (F=33.359,P=0.000).The expression quantityof GDNF in the4groups produced significant differences at7d(4.70±0.85,13.10±1.15,25.94±2.17and14.03±1.35, F=213.370, P=0.000),14d (5.82±1.23,24.41±1.43,25.96±1.49and25.83±2.46, F=163.459, P=0.000), and28d(4.95±0.88,26.66±2.25,25.83±2.46and25.18±1.60, F=194.002, P=0.000).Conclusion:The treatment of SGB at early stage of the facial nerve injury in rats may increase the expression of GDNF of the facial nerve nucleus at early stage, thus speed up the recovery of facial nerve injury.