Studies On Chemical Constituents And Quantitative Analysis Method For Total Saponins Of Trigonella Foenum-graecum L

In the first part of this dissertation, the research on chemical compositions of the total saponins of Trigonella foenum-graecum L. has been studied systematicly. In the second part, we have studied a method for quantitative analysis of the total saponins of Trigonella foenum-graecum L.. The third part, we organized domestic and foreign literatures, the structure and characteristics of NMR spectra of flavonoid glycosides and steroidal saponins compounds belonged to Trigonella foenum-graecum L. were briefly summarized.Trigonella foenum-graecum L.,which is known as "bitter beans, fenugreek, sweet beans, lemongrass, sweet clover", belongs to Fabaceae plants of the genus Trigonella, and the seeds have been used as medicine. Trigonella foenum-graecum L. has lots of steroidal saponins, flavonoid, alkaloids and others, most of them were steroidal saponins. Modern pharmacological research shows that the total saponins of Trigonella foenum-graecum L. has extensive hypoglycemic, hypolipidemic, anti-cancer, protect the myocardium, liver, anti-cerebral ischemia activity.In the first part of this dissertation, the chemical constituents of the total saponins of Trigonella foenum-graecum L. were studied systematicly.23compounds were isolated from the total saponins of Trigonella foenum-graecum L.(the80%ethanol percolate of the seeds of Trigonella foenum-graecum L., over D101macroporous resin column, eluted with70%ethanol, the resulting total saponin extract) and19compounds were identified by the spectroscopic methods and physicochemical property. Their structures were identified as palmitic acid (1), baohuoside I (2), methyl-a-D-glucoside(3), vitexin-2"-O-P-coumarate(4), isovitexin (5),vitexin(6),8-C-β-D-glucopyranosyl kaempferol(7), isoorientin(8), orientin(9), orientin-2"-O-P-coumarate(10), L-Tryptophan(11), soyasaponin I (12), methy trigoneoside I b(13), trigoneoside I b(14),(22S)-16β-[(β-D-glucopyranosyl)oxy]-22-hydroxycholest-5-en-3β-yl-O-a-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside(15), L-rhamnose (16), methy trigoneoside II b(17), methy protidioscin(18),(25R)-26-[(β-D-glucopyranosyl) oxy]-22a-methoxyfurost-5-en-3β-ylO-α-L-rhamnopyranosyl-(1→2)-O-[O-β-D- glucopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→3)]-β-D-glucopyranoside(19). Compounds1、2、3、7、11、13、15、16、17、19were all isolated from this plant for the first time.In the second part, by acid hydrolysis of Trigonella foenum-graecum L. total saponins, determination of the major hydrolysates of diosgenin contrent by HPLC, so as to establish a quantitative analysis method of indirect calibration of Trigonella foenum-graecum L. total saponins, provides reference for the content of effective components of Trigonella foenum-graecum L.. Samples were achieved and separated by Kromasil C18(200mm×4.6mm,5μm), with methanol-water (90:10) as mobile phase. The UV detector was sat at203nm. The column temperature was35℃. The result is that diosgenin was well separated. Through single factor and orthogonal test, effects of sulfuric acid concentration, hydrolysis time and amount of sulfuric acid were studied. The factors influencing hydrolysis-extraction efficiency were as follows: sulfuric acid concentration>reaction time>amount of sulfuric acid. The results showed that the optimization terms of hydrolysis conditions were:sulfuric acid concentration15%, hydrolysis time4h, amount of sulfuric acid40times.The third part, we organized domestic and foreign literatures, the structure and characteristics of NMR spectra of flavonoid glycosides and steroidal saponins compounds belonged to Trigonella foenum-graecum L. were briefly summarized.