Studies On Chemical Constituents Analysis And Preparative Technologies From Paeonia Paeoniae Pall

Traditional Chinese medicine composition was complex, various and different in content. How to rapidly and efficiently obtain the target group in traditional Chinese medicine in large size was a difficult problem in traditional Chinese medicine chemical research. Chromatography with its advantages of high efficiency and good repeatability becomes an ideal tool in compound preparation. This study which focused on industrial chromatography and used Paeonia Paeoniae Pall. as the subject discussed the sample pre-treatment technologies and chromatographic separation process parameters.UPLC/Q-TOF-MS was used to analyse the main components in Paeonia Paeoniae Pall. and nine kinds of possible structures were inferred. They were galloylsucrose, paeoniflorin sulfonate, albiflorin, paeoniflorin, galloylpaeoniflorinisomers, isopaeoniflorin or albiflorin R1, and benzoylpaeoniflorinWith TLC and HPLC as tracking detection means, Pre-treatments including extract, precipitation and the adjustment of pH in Paeonia lactiflora Pall, were studied. The results showed that extraction was a simple and effective pre-treatment technology and optimum extraction agent was ethyl acetate. A large number of other impurities were removed after AB-8macroporous resin enrichment. Chemical components was not change by precipitation The adjustment of pH value resulted in changes of chemical constructure of some composition which were bad for the systematic separations.Fr1.2from AB-8macroporous resin was selected as the research target. Guided by the TLC, a screening method was established including the elution system and solvent elution strength. Selectivity of best packing and solvents were obtained by the spots number and band tailing in TLC. The results showed that the best separation mode was silica gel. The mobile phase was chloroform-methanol (5:1). The best range of solvent strength for PLC judged with k was conformed from2to10. The analytes were separated completely when a was greater than or equal to1.5. The analytical chromatography obtained satisfactory result in the retention time. The maximum loading mass for the (4cm×40cm) preparative column was2.2g.