The Comparative Research Of Articular Surface Cartilage And Loose Body’s Surface Cartilage Using Fourier Transform Infrared Spectroscopic Imaging

Background and purpose：Taking the articular surface cartilage and loose body’s surfacecartilage as research objects,using Fourier Transform InfraredSpectroscopic Imaging and relative analysis to explore the different of thetwo samples which are in the same joint at the molecular level,furthermore to analysis the feature of biological behavior changing ofloose bodies’ surface cartilage.At the perspective of spectroscopy,discovering the relationship of loose bodies’ surface cartilage’ formativefactor and osteoarthritis, and exploring the discipline of growing anddifferentiation of articular cartilage and providing thought and theoryevidence for clinical therapy.Material and method：1. Clinical material all of samples’ resource is the patientsunderwent the operation of total hip/knee arthroplasty from Jan.2013toJan.2014,10case in the department of orthopedic of China-Japan UnionHospital of Jilin University,and loose body can be extractedintra-operative.1hip OA,9knee OA;4male,6female.the articularcartilage as control group comes from the same joint.All patients are examined by X-ray in order to make diagnose explicit;All samples arediagnosed by histopathology in order to ensure the difference betweencontrol and experimental groups.2. Method after samples are conducted,measurement were conductedwith the FT-IR imaging system in transmission mode.and then collectingcurves and making further conducting and calculating.3. Analysis：The second derivative IR peaks curves of two groups were calculatedwith help of SPSS19.0,and choosing the Collagen and PGs wavenumbersand their related regions of absorbance of all curves to form matrix tomake principle component analysis.Results：1.N-H bending vibration wavenumber of Type II collagen in thesecond derivative peaks in articular cartilage is1514cm-1,in the loosebody is1516cm-1,showing up an obvious blue shift.2.N-H bending vibration and C-N stretching vibration wavenumber ofAmide II in the second derivative peaks in articular cartilage is1573cm-1，in the loose body is1575cm-1,showing up an obvious blueshift.3.There is no different in the total PGs content of the two samplescalculated by an indirection method.4.There is different between articular cartilage and loose body surface cartilage in the scatter diagram composed by principal component axiscalculating by Principal ComponentAnalysis.Conclusion：1.the collagen in loose body surface cartilage is more stable than thecollagen in articular cartilage surface,it indicate that the collagen in loosebody surface as cartilage skeleton is hard to be retrogradation by relatedcollagenase.2.In the same joint fluid micro environment,the total PGs content,asthe stuffing,of articular cartilage and loose body surface cartilage is same.3.There is different in the component of collagen and PGs in the twosamples,the component of loose body surface cartilage has obviousdeficient compared with articular surface cartilage.