| Objectives In this study, we investigated the effects of TSA on osteoarthritis in rat chondrocytes induced by interleukin-1β and in a rat osteoarthritis model.Methods In the in vitro experiments, quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate changes in gene expression in TSA-treated chondrocytes with or without IL-1β treatment; changes in protein and acetylation levels were assessed by Western blot. For the in vivo study, TSA was administered by intra-articular injection in rats, after which gene expressions and protein levels were assessed. Macroscopic and histological observations were also evaluated.Results TSA inhibited IL-1β-stimulated MMP-1, MMP-3and MMP-13expression but upregulated TIMP-1expression and acetylation level of histones in cultured chondrocytes. In vivo, the same result can be concluded after the assess for gene expressions and protein levels. Besides, morphological and histological examinations demonstrated that TSA can ameliorated cartilage degeneration. Conclusion TSA has a potential therapeutic value for the treatment of osteoarthritis. |
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