Effect Of Deep Hypothermic Circulatory Arrest On Cytoskeletal Proteins Of MPTP In Hippocampus Neuron Of Rats

[Objective] To study the effect of deep hypothermic circulatory arrest (DHCA) oncytoskeletal proteins of mPTP in Hippocampus neuron of rats and mechanism of hypothermia in brain protection through inhibiting mitochondrial apoptosis.[Methods] Thirty-five SD rats were divided randomly into three groups:Deep hypothermic circulatory arrest (DHCA) group (n=10), Normal temperature circulatory arrest (NTCA) group (n=10) and Normal control (NC) group (n = 10), the next five rats were usedas the blood donor for other rats. The animal model of closed-chest DHCA was established in rats by means ofexternal cannulation. In DHCA group, ten minute’s circulatory arrest was performed after the temperature of rectum between 20℃-24℃ (by external circulation and body surface cooling); in NTCA group, ten minute’s circulatory arrest was performed in normal temperature by the same method; in NC group, ten minute’s external circulation without circulatory arrest. Hippocampus were dissociated after external circulation and then VDA、ANTand CYPD was measured by WB and RT-PCR, the results were analyzed by software SPSS 17.0. Data were expressed as the mean± standard error of the mean. Differences among groups were compared by one-way analysis of variance with post hoc comparisons performed using the Student-Newman-Keuls test of multiple comparisons. A difference of p<0.05 was considered significant.[Results] The levels of VDAC、ANT land CYPD expression of NTCA group and DHCA group were much higher than NC group (P<0.05). The levels of VDAC, ANT land CYPD expression of NTCA group were much higher than DHCA group (P<0.05)[Conclusions] Ischemia can induce the expression of VDAC、ANT and CYPD while deep hypothermia can reduce the expression apparently. Deep hypothermia can protect brain against injury induced by ischemia and anoxia through reducing the expression of VDAC、ANT and CYPD and inhibiting the open of mPTP.