| Objective: Qualitative to fucus yellow the extraction and purification process is optimized, and the preparation of high purity fucuxanthin.Method: Through the response surface analysis and orthogonal design method, qualitative to fucus yellow the extraction and purification process is optimized, and the purification of fucuxanthin samples by UV, HPLC, NMR structure identification.Results and conclusion: Determines the optimum process, the total by AB- 8 macroporous resin separation, purity can reach 40.2%, the silica gel column chromatography purification, purity can reach 84.71%, finally the preparation of thin layer chromatography further purification, the purity of 96.7%, and the structure identification of purified compounds was confirmed as fucuxanthin. |