The Effects Of Flavonoids From Coreopsis Tinctoria On Decreasing Blood Pressure, Lipid And Viscosity

Cardiovascular and cerebrovascular diseasesdisease is the number one killer threatening human health. High blood pressure, high cholesterol, high blood viscosity are important risk factors for cardiovascular and cerebrovascular diseases caused in recent years and the incidence rate has be gradually getting younger and younger. Coreopsis tinctoria contains a variety of active ingredients and the most abundant is flavonoids. However, the main study of Coreopsis tinctora is priority to aspect about chemical composition meanwhile the research of pharmacological effects is rare currently. Therefore, the study of Flavonoids from Coreopsis tinctoria(FCT)lowering blood pressure, blood lipids, blood viscosity for the adjuvant treatment of cardiovascular disease have a positive significance in reducing the incidence of cardiovascular and cerebrovascular disease and secondary treatments. It has also providing theoretical basis for the development and utilization of Coreopsis tinctora resources.Method Ultrasonic synergy ethanol reflux extraction method was used to extract the FCT and FCT component determination was using UV spectrophotometry and HPL in this study. The influences of FCT to arterial blood pressure and the tension of vascular rings were observed by the method of acute in vivo and in vitro experiment in rabbits. The effects of FCT on plasma related indicators such as total cholesterol(TC), total glycerin(TG), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C)and hemorheology at of hyperviscosity rats were researched as well.Results Optimal conditions for the extraction of FCT is follow: 60 minute extraction time, 20 m L/g solid-liquid ratio, 60℃ extraction temperature, 100 W ultrasonic power. Maximum extraction rate was 19.65%. The extract contains rutin and other more than two flavonoid components analyzed by HPLC ultrasonic synergy.FCT can effectively reduce blood pressure of rabbits and diastolic blood vessels pretreated by NE with dose dependent density(P<0.05 or P<0.01). L-NAME, methylene blue and indomethacin pretreatment can inhibit the effects of the FCT reduction of vascular tone(P<0.05 or P<0.01). It is suggested that the effect of FTC might related to vascular endothelial cells by activating endothelial cells release NO, enhancing guanylate cyclase activity. Furthermore, FTC could diastole vascular smooth muscle by activating endothelial cells release prostaglandin I2(PGI2), inhibiting Ca2+ of intracellular transportation.Intra-gastric of FTC in rats for 4 weeks caused the decrease of plasma TC, TG, LDL-C and blood glucose levels and the increase of HDL-C on the contrary(P<0.05 或 P<0.01).Intra-gastric FTC for 10 days in rats could decrease shear rate of the whole blood viscosity(1s-1、5s-1、30 s-1、200 s-1), Casson’s viscosity, yield stress, flo resistance, the relative index(high, medium and low-cut rate), plasma viscosity,erythrocyte viscosity, erythrocyte aggregation indexs at acute hyperviscosity rat model induced by epinephrine(P<0.05 或 P<0.01).Conclusion Significantly, FTC has the effects on lowering blood pressure, reducing vascular tone and decreasing plasma levels of TC, TG, LDL-C and blood sugar, instead, increasing the levels of HDL-C, and improving rheology indicators of blood at hyperviscosity rat model.