Study On The Expression Of CRM1 MRNA In Multiple Myeloma KM3 Cell Line By Using Arsenic Trioxide

Objective: The aim of this study was to investigate the expression of CRM1 gene mRNA in multiple myeloma KM3 cell line by using Arsenic trioxide。Methods: Cell proliferation was observed by the Cell Counting Kit-8; Using inverted microscope to observe the cell morphology after the drug and to take a photograph. The cell apoptosis was analyzed by flow cytornetry. To determine the mRNA expressions of CRM1 in KM3 cell line after using different concentrations of As₂O₃ by RT-PCR;Results: Arsenic trioxide inhibit the proliferation of KM3 cells with obvious dose effects; The apoptosis rates for 10、20、30μmol/L As₂O₃ after 24 h in KM3 cells were（23.95±0.212）%（24.30±1.698）%（42.15±0.4949）% respectively in KM3 cells. With the control group（5.15±0.212）%,there were significant differences（P<0.05）;Compared with control group, the mRNA expression was reduced in As₂O₃ groups（p<0.05）.No significant difference was observed between the every experimental groups.Conclusion: As₂O₃ can inhibiting KM3 cell proliferation and induce cell apoptosis;CRM1 mRNA expression was decreased.