The Mechanisms Of Panax Notoginseng Saponins And Breviscapine In Preventing Skin Photoaging And Comparison Study

ObjectiveTo establish Balb/c animal model of mice’s skin photoaging, and study the effects of local application of Panax Notoginseng Saponins (PNS) and breviscapine to the TEWL value changes in Balb/c mice’s photoaging skins due to chronic exposure to ultraviolet (UV); study the effects of PNS and breviscapine to the MMP-1 expression changes in Balb/c mice’s photoaging skin tissues; study the effects of PNS and breviscapine to the IL-la and IL-6 content changes in Balb/c mice’s photoaging skin tissues; discuss the mechanisms of PNS and breviscapine in preventing skin photoaging, compare the advantages and disadvantages, and provide theoretical foundation for prevention and treatment of photoaging and development of skin care products.MethodDivide 50 healthy female Balb/c mice randomly into 10 groups:control group (Group A), UV group (Group B), matrix group (Group C), positive control group (Group D model+0.3% tea polyphenol), experimental group (Group E model+0.05% PNS, Group F model+0.1% PNS, Group G model+0.2% PNS, Group H model+0.05% breviscapine, Group I model+0.1% breviscapine, Group J model+0.2% breviscapine). Simulate UV radiation (UVA and UVB) in the sun, establish animal model of photoaging, before radiation, apply 0.1 g matrix gel,0.3% tea polyphenol gel,0.05%,0.10% and 0.2% PNS gel,0.05%,0.1% and 0.2% breviscapine gel respectively on the radiation back of Group C, D, E, F, G, H, I, J. After 9 weeks of radiation and application of samples, adopt non-invasive skin test method to detect the TEWL changes, adopt immunohistochemical method to detect expression levels of MMP-1, adopt enzyme-linked immunosorbent assay (ELISA) method to detect the secretion levels of IL-1a and IL-6.ResultAfter 9 weeks of UVA and UVB radiation, the skins of the experimented place of Group B Balk/c mice show photoaging charateristics like erythema, desquamation and wrinkles, so the establishment of animal model is successful. After 9 weeks of UVA and UVB radiation and local application of samples, the detection results of relative indexes of each group are as below:1. through non-invasive skin test, TEWL value of Group B is 5.95±0.37g/cm2·h, more that the valves of experimental group and Group D, the differences between Group E, G, H, I, D and Group B show statistic significance (P<0.5), illustrating the role of repairing skin barrier function. Group G is the best.2. through immunohistochemical test, the expression results of MMP-1 of Group B is strong positive, Group E positive, Group F positive, Group G weak positive, Group H positive, Group I weak positive, Group J positive and Group D weak positive, the expression of MMP-1 in experimental group and Group D all lower than Group B, the differences between Group E, F, G, H, I, D and Group B show statistic significance (P<0.5), illustrating the role of suppressing the expression of MMP-1.Group G is the best.3. Through ELISA test, the IL-la content and IL-6 content of Group B are respectively 164.1±3.8ng/L and 210.6±1.2ng/L, more than those of Group D and experimental group, the differences between Group E, G, H, I, D and Group B show statistic significance (P<0.5), illustrating the role of decreasing the contents of IL-1α and IL-6. Group G is the best.ConclusionExternal use of Panax Notoginseng Saponins and breviscapine gelatin can repair UV-induced Balb/c mice’s photoaging skin barrier function, down-regulate levels of inflammatory cytokines and inhabit MMP-1 expression thus to prevent mice’s skin photoaging. Among them, high concentration group of Panax Notoginseng Saponins achieves best effect of preventing skin photoaging. The research provides experimental foundation for medical grade anti-aging skin care products.