Apolipoprotein E Polymorphism Influence On The Prognosis Of Patients With Spinal Cord Injury

Objective:To investigate the influence and the correlation of apolipoprotein E (ApoE) gene polymorphism on the prognosis of patients with spinal cord injury.Methods:1. Using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) and gene sequencing method, from March 2014 to February 2015 for treatment of spinal cord injury in 50 cases, the Second Affiliated Hospital of Kunming Medical Rehabilitation Division ApoE genotyping patients;2.Aaccording to the degree of damage to the patient’s spine and limb function stable condition and other specific circumstances, all patients comprehensive rehabilitation therapy, the use of drugs, neurotrophic and microcirculation therapy, breathing exercises, strength training, stretching, endurance training, balance training, joint range of motion training, transfer of training, operation and wheelchair skills training, training in activities of daily living, such as rehabilitation, for different injuries in different segments of the orthopedic patient wears a limb with treatment.The frequency of treatment:once a day,5 times a week;3. The patients were evaluated according to ASIA classification of spinal cord injury. Patients were evaluated respectively at admission, after 1 months of treatment, ASIA was observed in the degree of spinal cord injury, light touch, pinprick score, score and activities of daily living (FIM) changes etc.4. Using SPSS 17.0 software changes ApoE genotyping results and the results of the functional assessment analysis and the influence of ApoE gene polymorphism on the effect of spinal cord injury rehabilitation.Results:l.The actual collecting cases 47 cases, ApoE genotype test results:ApoEε3/3 type most frequently (68%), ε2 / 3 (15%) and ε3/4 (11%), followed by, ε4/4 (2%) and ε2 / 2 minimum, patients ApoEs4 carriers eight cases (17%), non-carriers 39 cases (83%), ApoE genotype consistent with Hardy-Weinberg law, in line with the gene distribution of natural populations.2. ASIA score change:after all patients before treatment and 1 month of treatment compared to light touch, pinprick and sports scores have improved, the difference was statistically significant (P<0.05); sports scores, ApoEε4 carry group recovery ε4 non-carrying group somewhat less than that, the difference was statistically significant; and light touch and pinprick recovery, the difference was not statistically significant between the two groups (P> 0.05).3. Activities of daily living (ADL) Rating:FIM scores compared two groups of patients before treatment were increased in January, ApoEε4 ε4 carriers than the noncarriers recovery is poor, the difference between the two groups was statistically significant (P< 0.05).Conclusion:1. Neurological function in patients with spinal cord injury rehabilitation and ApoE gene polymorphism. This study can provide the basis for the future development of gene therapy for spinal cord injury.2. Carrying motor function in patients with activities of daily living ApoEε4 of recovery is poor, there was no significant difference in the recovery of light touch and pinprick aspects ApoEε4 carriers and non-carriers.3. In the clinical course of therapy, spinal cord injury patients ApoEε4 allele carries its attention to strengthening the rehabilitation of motor function, exercise capacity and promote the recovery of their daily living.