Study On Correlation Of TGF-β In Excessive Muscle Fibrosis By Traumatic Osteomyelitis

Objective According to the traditional animal models,to establish a closer clinical rat model of traumatic osteomyelitis, and to explore survival rate and incidence of infection of animal at different concentrations of Staphylococcus aureus suspension. To explore mechanism of skeletal muscle excessive fibrosis in traumatic osteomyelitis.Methods 70 healthy male rats were randomized into 3 groups:control group and experimental Groups A(106CFU/ml bacteria solution),B(102CFU/ml bacteria solution).There were 30 rats in every experimental group and 10 rats in control Group C. To establish traumatic osteomyelitis model, the severity of experimental Staphylococcus aureus osteomyelitis in rats was evaluated so that optimal bacterial concentration can be known. 90 healthy male rats were randomized into 3 groups:Experimental groups(102 CFU/ml bacteria solution), sham operation group(normal saline), 40 each group, and 10 in control group. histopathologic changes and the expression of TGF-β of surrounding tissues in different time was detected with HE staining and immunohistochemistry.Result By observation and evaluation of index of Statistical results, infection rates of Group B(102CFU/ml) approach to Group A(106CFU/ml), with the lowest mortality. HE staining: fibrosis of Group A higher than Group B during the moulding period. Immunohistochemistry: From the 7th day, expression of TGF-β of Group A is gradually increased. At days 28 after surgery, expression of TGF-β keeps a high level.Conclusion On the basis of the past traditional model, a new osteomyelitis model which simulate severe soft tissue trauma, fractures, bacterial infections, internal fixation surgery can be got.After the formation of osteomyelitis in rats, skeletal muscle fibrosis is closely related to the expression of TGF-β.