| ObjectiveTo study the effect of Ailuo Kechuanning oral liquid on Lung function and interleukin-8(IL-8),Tumor neerosis faetor-alpha(TNF-α),Macrophage inflammatory protein-2(MIP-2) levels in plasma,lung tissue homogenate and bronchial alveolar lavage fluid(BALF) and lung tissue pathology,mucin 5AC(MUC5AC),water channel protein 5(AQP5) gene expression in pulmonary qi deficiency rats with Chronic Obstructive Pulmonary Disease.Explore Ailuo Kechuanning treatment for COPD lung deficiency and its mechanism of action, provide experimental evidence for the clinical application of Ailuo Kechuanning parties for the development of traditional Chinese medicine treatment of COPD to provide new ideas.MethodsThe 60 Wistar rats were randomly divided into six groups, namely normal control group, model group, Ailuo Kechuanning low(7.76 g · kg-1 · d-1), medium(15.52 g · kg-1 · d-1), high(31.04 g · kg-1 · d-1) dose group, acute bronchitis syrup(3ml · d-1) groups of 10. To within plus smoked intratracheal instillation of Lipopolysaccharide(LPS) preparation of stable COPD lung deficiency rats were given corresponding drugs; observe and record the general condition of rats to detect lung function and coverage. Enzyme-linked immunosorbent assay in plasma, lung tissue and bronchoalveolar lavage fluid in rats with IL-8, TNF-α, MIP-2 content, the light microscope structure of lung tissue, in situ hybridization and immunohistochemistry MUC5 AC staining in lung tissue, AQP5 mRNA and protein expression. Related to the experimental data using SPSS13.0 statistical software for processing. ResultsThe study was prepared in stable COPD lung deficiency rat model to determine lung function and pathological changes in rat model as the basis for successful replication. Compared with the normal group, tidal volume(TV) model rats, peak expiratory flow(PEF), 50% of tidal volume expiratory flow(EF50) were significantly lower than normal group(P <0.05 or P <0.01); plasma IL-8, TNF- and MIPɑ-2 factor were significantly higher than the normal group(P <0.05 or P <0.01), lung tissue IL-8, TNF- ɑand MIP-2 were significantly higher factor normal group(P <0.05 or P <0.01), bronchoalveolar lavage fluid IL-8, TNF- ɑand MIP-2 factor were significantly higher than the normal group(P <0.05 or P <0.01); the trachea and the model group MUC5 AC mRNA and protein expression in lung tissue were significantly enhanced significantly weakened AQP5 mRNA and protein expression, protein expression in model group and AQP5 MUC5 AC protein was negatively correlated(trachea r=-0.528, P <0.01; lung r=-0.532, P <0.01). Compared with the model group, Ailuo Kechuanning low, medium and high dose group and acute bronchitis syrup rats TV, PEF and EF50 were significantly higher(P <0.05 or P <0.01); plasma IL-8, TNF- ɑand MIP-2 factors were significantly lower than the model group(P <0.01), lung tissue IL-8, TNF- and ɑMIP-2 factors were significantly lower than the model group(P <0.05 or P <0.01), BALF of IL-8, TNF- ɑand MIP-2 factors were significantly lower than the model group(P <0.01); love MUC5 AC mRNA and protein expression in Ailuo Kechuanning medium dose group trachea and lung They were significantly reduced, AQP5 mRNA and protein expression were significantly increased, the middle dose group and AQP5 MUC5 AC protein expression of negative correlation(trachea r =-0.485, P <0.05; lung r =-0.491, P <0.01). Model group exudate within bronchial lumen, bronchial epithelium with multiple degeneration, necrosis, inflammatory cell infiltration of the wall layers significantly; submucosal mucous gland hyperplasia, expansion; a large area surrounding the bronchioles and alveoli appear inflammatory cell infiltration, showing the limitations of atelectasis, bullae appear around the lesion. Compared with the model group, pathological changes in the Ailuo Kechuanning low, medium, high dose group and acute bronchitis syrup group of relatively light, medium dose group repair the most obvious. Conclusions1 Ailuo Kechuanning can strengthen the function of rat lung ventilation, and air, improve the symptoms of COPD stabilization of rat lung qi deficiency. 2 Ailuo Kechuanning would significantly reduce inflammation, COPD lung tissue injury, improve the symptoms of lung qi deficiency. Its mechanism by inhibiting cytokines IL- 8, TNF- ɑ and MIP- 2 secretion, prevent the development of inflammation. 3 Ailuo Kechuanning can resistance to high airway mucus secretion, with phlegm, and expectorative effect. The mechanism by cutting MUC5 AC, raised AQP5 gene expression, adjust mucus sticky protein and water in the proportion. 4 Ailuo Kechuanning can significantly reduce the COPD stabilization of rat lung deficiency of phlegm, asthma and other symptoms. Its mechanism may be related to airway inflammation and mucus secretion of high resistance. |
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