Study On Lipid Metabolism Related Enzymes By PPAR Regulation Of Soybean Three Hydroxy Isoflavone

Objective:Soybean isoflavone is a secondary metabolites leguminous plants such as soybeans, Gen is an important active component of soybean isoflavones, the study found that its regulating lipid metabolism, anticancer, antioxidant and so on the many kinds of biological functions. Show the regulation of metabolism in lipid metabolism enzyme expression and activity of physiological functions. In this article, through cell in vitro experiments to study Genistein(Gen) effect on the regulation of lipid metabolism, and Gen to phosphorylation amp activated protein kinase (AMPK) content, the influence of the peroxidase body growth activated receptors (PPARs) in Gen regulating lipoprotein lipase (LPL) gene expression in the function and way to activate the mechanism of the law, for soybean isoflavone in nutrition health care, prevention of the metabolic syndrome, clinical pharmacology, and other fields of applied research provides the theoretical basis and experimental basis.Method:We take four kinds of cells (L-02, HepG2, Hep3b,3T3-L)as research object, these cells were divided into control group, model group, Gen experimental group, positive control group (PPARs agonists).Model group which OA induced was model control. To determine the optimal dose of OA, Gen and PPAR agonists were treated on four kinds of cells and then detected different concentrations of OA-induced cells activity by MTT method. Testing the contents of ALT and AST after different cells were treated by OA in different time to determine the best time of OA role and then used the optimal dose and optimal time parameters to establish adipocyte model. Evaluating the effects of Gen and PPAR agonist regulate on TG by detected TG content after OA-induced cells. ELISA method was used to detect the content changing of AMPK in four different kinds of cells culture medium after Gen and PPARS treated. The mRNA expressions of LPL, LXR and PPARa, PPARy in different cells were detected by real-time PCR respectively and the protein contents of PPARa, PPARy were determined by Elisa Kits.Results:(1) We conclude that lmM OA treated four different kinds of cells at 48 h is the best combination to establish fat cell model by OA induced.(2) Gen can increase the AMPK concentration and also it was dose-dependent. Gen showed consistent direction with PPARa, PPARy agonist (3)Genistein can up-regulate the mRNA expression of LPL and PPARa, PPARy in a different degree and increase the protein content of the PPARa and PPAR γ Genistein can performance consistent results like PPARa and PPARy agonist.Conclusion:Gen fat cells caused by OA has certain regulation effect, and this may be related to Gen via PPARs raised the main related metabolic TG enzyme AMPK and LPL expression, increase the AMPK and LPL activity to promote the cell to TG fat metabolism. Activate PPARs way can increase the LPL and orphan nuclear receptors in the cell of PPAR alpha, PPAR gamma mRNA expression Gen to a certain extent, increased the protein content of PPAR alpha and PPAR gamma, Gen may fat cells by activating PPAR (mainly PPAR gamma) to adjust the concentration of the AMPK and LPL mRNA expression, promote fat metabolism of intracellular TG, reducing fat on the role of cell damage.