The Research On The Fenofibrate Ovalbumin Nanoparticles As Oral Drug Delivery System

Fenofibrate is a kind of antilipemic drug which is widely used in treating high cholesterol, hypertriglyceridemia and other combined hyperlipidemia. According to the biopharmacy classification system, fenofibrate was classified as class II of the BCS due to its low solubility and high intestinal permeability, which limits its widely application at the field of medicine. Literatures often reported that nanosuspensions were used to improve the bioavailability of fenofibrate, but it often contains lots of surfactant and brings some side effect to human body. As a result, finding an easy and safe strategy to improve the bioavailability of fenofibrate has very important significance to improve the bioavailability of fenofibrate.Oral drug delivery system can greatly improve the dissolution of poorly soluble drugs, which is the highlight of pharmaceutics. Natural polymer proteins such as collagen、casein、ovalbumin、gelatin is degradable and biocompatibility, which is widely applied in drug delivery.In this study, we use ovalbumin to prepare fenofibrate loaded nanoparticles, and we use mean particle diameter as the main evaluation index. The experiments such as particle size, size distribution, electron microscope analysis, X-ray diffraction and in vitro dissolution are used to analysis the optimized process combination.The physicochemical characteristic of ovalbumin was studied. The optimized drug loading is 20%, while the average particle size is 432nm and the polydipersity index is 0.029.The scanning electron microscope spectrum indicated that homogenous particle size distribution with some spherecity particles and particle size ranges from 300-500nm. X-ray powder diffraction (XRD) and differential (DSC) we performed to performed to analyze potential changes of its crystalline transfer. The results demonstrated that fenofibrate were in amorphous state incorporated in ovalbumin nanoparticles,and no obvious crystal diffraction peaks were observed.We established fenofibrate in vivo determination of content of chromatographic conditions. The in vivo test showed that fenofibrate loaded nanoparticles can enhance the bioavailability of ovalbumin compared with fenofibrate raw materials.