| Objective : To investigate the protective effect of L-carnitine valproate-induced acute liver injury in young rats.Methods: VPA induced in young rats with acute liver injury induced by different doses of L-carnitine and biochemical indexes contrast change, alter liver antioxidant index and pathology before and after treatment. Establishment of acute liver injury model of rats induced by sodium valproate,the rats were randomly divided into 5 groups,normal group, model group,and three groups of LC(0.25, 0.5g, 1.0g/kg). The normal group was given equal volume of distilled water, the model group gavage of sodium valproate 100mg/kg, LC groups were gavage of sodium valproate in 100mg/kg, one group received LC0.25g/kg, second group were given LC0.5g/kg, the third group was given LC1.0g/kg, administered 1 times daily, for 21 days. At 24 h after the last administration, rat tail vein blood 2ml.,using ALT and AST kit and OLYMPUS multi function automatic biochemical analyzer for the determination of biochemical index in serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) level. Detection of liver tissue, using the method of xanthine oxidase and superoxide dismutase(Superoxide, Dismutase, SOD) activity and malondialdehyde(MDA) content. The anatomy of rat liver tissue, and pathological observation of liver tissue of eye view of change part of the liver tissue was taken and fixed in 10% formalin, embedded in paraffin, sliced and stained with HE, observed the pathological changes of liver under light microscope.Results: Successfully established the liver pathological damage model induced by sodium valproate. Model group compared with the normal group, the serum levels of ALT, AST were increased significantly. After oral administration of LC in acute liver injury animal serum ALT, AST were decreased, in a dose-dependent manner(P1>0.05,P2、3<0.05), the level of SOD in liver tissues of rats were increased dose dependently(P1>0.05,P2、3<0.05), and the content of MDA increased with the dose of LC decreased(P1>0.05,P2、3<0.05), reduce the pathological changes of liver tissue of rats effectively.Conclusion: The protective effect of LC on liver injury induced by VPA in the range of 0.25-1.0g/kg, the mechanism of its protective effect should be related to its antioxidant action. |
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