| Objectives To observe the inhibitory effect on candida albicans and the tensile strength of denture adhesive of a mixture which made of water-soluble chitosan and different kinds of traditional Chinese medicine composition, it can explore a new preparation which can combats the bacteria and being denture adhesive. It provide a experimental basis for people to develop a new denture adhesive.Methods 1 The preparation of water-soluble chitosan: mixture concentration of 2%(2mg) in chitosan add in 100 ml of distilled water solvent, in each, all needs to make a new solvent. 2 The preparation of traditional Chinese medicine(TCM): put 100 g traditional Chinese medicine respectively in 800 ml distilled water for half an hour, after keep the decoction micro boiling by Slow fire for 2 h, filter the decoction.Then boil the remain medicine with appropriate water for 1 h and filter it.Merge the two filtrates, concentrate and drie it, then mix the extract with Paris polyphylla to make the traditional Chinese medicine polyphylla chitosan mixture. Grouping is: the blank group(group A), the control group(group B), Paris polyphylla-chitosan group(group C), cortex phellodendri-chitosan group(group D), rhiaoma polygoni cuspidati-chitosan group(group E), radix scutellariaechitosan group(group F), cortex cinnamomi-chitosan group(group G), cortex dictanmnichitosan group(group H). 3 Measure the bacteriostatic test and determination of MIC values: Sabouraud agar medium disinfected in high temperature,when the temperature dropped to 45 ℃, took the Sabouraud agar medium mix with 1 ml candida albicans which has deliquate to 1×108 cfu/ml, and pour it into 9 cm inner diameter sterilization AGAR, to set. In this way make several AGAR. Plate can be divided into four areas, the Oxford cup(inner diameter 6 mm, 8 mm outside diameter, and high 10 mm) light on the corresponding position, respectively, to join with good mixture(add up to Oxford cup 4/5 level), with the blank group and nystatin as positive control and negative control respectively. Plate to be included in the constant temperature incubator, 37 ℃ for 24 h, and inversion of AGAR,with the naked eye can see clearly not of fungal growth area for the edge of the bacteriostatic circle, from four different directions by scale measuring the diameter of bacteriostatic ring, calculate the average.Experimental repeat 5 times, averaging. By the double dilution method reagent dilution into different concentrations, MIC value with the above method test. 4 Measurement of denture adhesive’s tensile bond strength: preparation with poly(methyl methacrylate) 0.2 cm×2 cm×5 cm specimen 60, above the specimen with the grip, will Chinese medicine- chitosan mixture evenly coated in specimens of adhesive surface, 2 for a group of soaking in artificial saliva, took out a set of every 1hours, fixed in electronic universal tensile test machine. Start the instrument, two specimen slowly contact, pressure keep the 30 s, again start the instrument, with tensile speed of 50mm/min to make two specimens, slow separation and record separate two specimens of maximum breaking load(N). After test in each group, with 75% alcohol and distilled water to clean specimen, according to the above steps to repeat the experiment Calculation of bonding tensile strength: bonding tensile strength(TBS) = F/S, F(N), adhesion to S for the specimen on the surface of the bonding surface area(cm2); S=10 cm2, each group of recipe test 5 times, take the average.Results 1 Paris polyphylla, cortex phellodendri and radix scutellariae of six kinds Chinese herbal medicine has bacteriostatic action to CA. Under the the same concentration,Paris polyphylla’s inhibitory effect on candida albicans is better than cortex phellodendri,radix scutellariae,and the difference was statistically significant(F value were 244.351,71.978 and 244.351, P<0.05). The cortex phellodendri and radix scutellariae bacteriostatic effect is no statistical difference. Rhiaoma polygoni cuspidati, cortex cinnamomi and cortex dictanmni have no bacteriostasis to CA. 2 The MIC of Paris polyphylla is 1.563mg/ml. 3 The tensile bond strength value of Paris polyphylla-chitosan mixture at 0 h is greater than the Protefix denture adhesive, between the difference was statistically significant(t=5.777, P<0.05); After immersed in artificial saliva at 5 h, the maximum of tensile bond strength value of Paris polyphylla-chitosan mixture is greater than the maximum of Protefix denture adhesive values, between the difference was statistically significant(t=14.372, P<0.05); After immersed in artificial saliva at 12 h, the tensile bond strength value of Paris polyphylla-chitosan mixture is greater than the maximum of Protefix denture adhesive values,between the difference was statistically significant(t=3.673, P<0.05). Determination of Paris polyphylla-chitosan mixture in the process of soaking in artificial saliva for 12 h intervals of 1 h adhesive tensile strength value is greater than the Protefix denture solid agent, the t test showed that in the two groups during the period of preparation for 12 h bonding tensile strength mean difference was statistically significant(t=2.103, P<0.05).Conclusions 1 Traditional Chinese medicine Paris polyphylla chitosan-mixture has good inhibition effect on Candida albicans. 2 Traditional Chinese medicine Paris polyphyllachitosan mixture has good adhesive tensile strength. 3 Traditional Chinese medicine Paris polyphylla-chitosan mixture has the clinical characteristics as denture adhesive. |
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