Changes Of Endoplasmic Reticulum Stress-related Molecules GRP78 And CHOP In Rat Severe Acute Pancreatitis-associated Liver Injury

Objectives To observe the changes of endoplasmic reticulum stress-related molecules glucose-regulated protein 78(GRP78) and CCAAT/enhancerbinding protein homologous protein(CHOP) in the severe acute pancreatitis-associated liver injury. Discussed whether endoplasmic reticulum stress(ERS) participate in the progress of severe acute pancreatitisassociated liver injury.Methods The subject established the model of severe acute pancreatitis-associated liver injury by 5% sodium taurocholate. 80 male SD rats were randomly divided into sham groups(n=40) and model groups(n=40). Then each groups were randomly divided into 3h,6h, 12 h, 24 h and 36 h according time points. The changes of serum amylase(AMY),alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected by automatic biochemical analyzer. Pathological changes of the liver were observed with HE staining. The expression of GRP78 and CHOP were detected by Western blot.Results 1 The level of serum amylase, alanine aminotransferase and aspartate aminotransferase were significantly increased and reached the peak value at 12 h in model groups(P<0.05). The level of serum amylase, alanine aminotransferase and aspartate aminotransferase in the model groups were significantly increased compared with corresponding time points of sham groups(P<0.05). The level of serum amylase, alanine aminotransferase and aspartate aminotransferase were no obvious change in sham groups(P>0.05). 2 The HE staining showed that the injury of the liver was the most serious at 24 h.Liver cells were gradually edema. Liver sinus were gradually expanded,extravasated blood and inflammatory cells infiltration. 3 Western blot test showed that the expression of GRP78 and CHOP in the model groups were significantly increased and reached the peak value at 24h(P<0.05). The expression of GRP78 in the model groups(3h,6h, 12 h, 24 h and 24h) were significantly increased compared with corresponding time points of sham groups(P<0.05). The expression of CHOP in the model groups( 6h, 12 h,24h and 24h) were significantly increased compared with corresponding time points of sham groups(P<0.05). The expression of GRP78 and CHOP in the sham groups were no obvious change(P>0.05).Conclusions The injury of the liver was the most serious at 24 h and ERS may be involved in the progress of severe acute pancreatitis-associated liver injury.