Research On The Relationship Between Brain-specific Proteins And Brain Edema In Acute Cerebral Infarction

Objectives Observe the change in concentration of serum neuron-specific enolase(NSE)and S-100 b protein in patients with acute cerebral infarction, at the same time,application of noninvasive brain edema determination of dynamic monitor patients with lateral hemisphere cerebral electrical impedance disturbance factor(CEI) to determine the severity of the patients with cerebral edema. To analyze the relationship between the levels of serum NSE, S-100 b protein and the severity of cerebral edema. In order to provide new indicators for early detection and assessment of severity of brain edema and clinical medicine.Methods Select 120 cases of patients with acute cerebral infarction onset within 2d, 80 cases from outpatient with healthy as research subjects. In cerebral infarction group, blood samples at the elbow vein were collected during the fasting state in the morning, after the onset of the first 2,3,5,7d. And in control group, fasting blood samples were collected only once, and the approach of specimen processing same as cerebral infarction group. The protein concentrations of serum NSE, S-100 b from two group were measured by ELISA.At the same time, using BORN-BE measure the CEI of the affected side hemisphere, in strict accordance with operating specifications, for determining the severity of patients with cerebral edema. To analyze the relationship between the levels of serum NSE, S-100 b protein and the severity of cerebral edema in patients with acute cerebral infarction. All date was analyzed using ANOVA or Chi-square test, Linear correlation analysis by SPSS17.0 software(P<0.05).Results 1 Patients with acute cerebral infarction group compared with control group,serum NSE and S-100 b protein levels were significantly increased after admission 2d,protein concentration gradually increased following days, the first three days higher than the first two days and the first five days higher than the first three days, serum protein concentrations reach a peak of about the first five days, but it is in the first seven days decreased, the difference was statistically significant(P<0.05). 2 The patients have a substantially consistent trends in the the affected side CEI values and NSE, S-100 b protein concentrations in the cerebral infarction group. Brain edema gradually increased after the onset, its severity was positively correlated with serum NSE and S-100 b proteinconcentration(P<0.05). 3 Serum NSE and S-100 b protein concentration compared with each other in patients with different infarct size. At the same time, their concentration in large infarction group was higher than in the middle area of infarction and the middle infarct group was higher than that of small area infarction. It showed that serum NSE and S-100 b protein concentration is increased with cerebral exacerbation and the patients with different infarct area of three groups have a substantially consistent trends in the the affected side CEI values and NSE, S-100 b protein concentration. The difference was statistically significant(P<0.05). 4 Progressive cerebral infarction group compared with complete cerebral infarction group, the serum NSE and S-100 b protein in progressive cerebral infarction group reach to a peak time of concentration is longer than the complete infarction group. In complete cerebral infarction group, the time of reached the peak is in the first 3-5 days, and it is in the first 5-7 days in progressive cerebral infarction group. In addition, the patients of two groups have a substantially consistent trends in the the affected side CEI values and NSE, S-100 b protein concentration. the difference was statistically significant(P<0.05).Conclusions Serum NSE and S-100 b protein levels were increased in the acute stage,and showed dynamic changes along with the progression. It is closely related to progression of cerebral edema and has some clinical guidance value in judging the severity of cerebral edema. It promises to be a new laboratory parameters in noninvasive methods to detect the extent of cerebral edema.