A Preliminary Study On The Quality Control Of Cortex Moutan Charcoal And Its Metabolomics

Moutan is Ranunculaceae peony(Paeonia suffruticosa Andr.) Dried root bark. bitter, acrid, slightly cold, with heat and cooling blood, blood stasis effect, for Yin fever, bleeding amenorrhea. Clinically heat and cooling blood circulation function, commonly used in heat penetrating the blood, warm spot poison embolism, for no sweat Gu Zheng, amenorrhea, sore also have a certain effect. Moutan carbon is potency cold and taste octyl, into the heart, liver and kidney. Having cooling hemostasis, can be used for vomiting blood, blood heat uterine bleeding and many other card, Peony charcoal after its ease cold of nature, enhanced hemostatic effect, used to heat the blood and bleeding. Moutan fried carbon after being elected by the opposite effect: cooling blood→cooling bleeding, change its inevitable effect of the medicinal ingredient and process-related effects in vivo. Therefore, this project from moutan charcoal processing technology, quality control and metabolic aspects of genomics research.The first, Research on processing technology moutan charcoal cooked. Take the Box-Behnken response surface methodology to optimize the charcoal Cortex Moutan best processing technology. When cooked processing method using river sand of hot method, using gallic acid, 5-HMF, catechin content of paeonol four kinds of substances as an evaluation index score. Use concocted processing time, processing and processing temperature, the amount of river sand, these three as a single factor experiment; then, on this basis, takes Box-Behnken response surface methodology to optimize the best charcoal Cortex Moutan Processing Technology. Finally, the best way to Cortex Moutan charcoal cooked processed is: concocted processing time is 9.0 min, concocted processing temperature is 180.0 ℃, adding the proportion of river sand is 25.5. The content of the prediction values of the four component scores of the response value is 95.081; Found average value is 94.314, close to the predicted value.The second. Research on moutan charcoal quality control.Blue pigment adsorption and phosphomolybdic acid colorimetric carbon moutan be determined by application of methylene give moutan pigment carbon adsorption of methylene blue on the proposal of not less than 7.7 mg.g-1, recommendation tannin content of not less than 0.81 mg.g-1. By ultraviolet spectral line group and the absorption peak number and peak position of ultraviolet spectral line group moutan and moutan charcoal cooked to varying degrees and ultraviolet spectral line profiles of different polar fractions were studied, and the results processed products moutan absorption of ultraviolet spectral line group and the absorption peak position of having a significant difference. Determination of varying degrees of processing carbon moutan its drug of 5-HMF by using HPLC method, not acid, catechin, gallic paeonol found, gallic acid and 5-HMF with increased processing time extended, but began to decline after a certain degree; catechins and paeonol decreased.The third, Metabolomics studies after administration of charcoal Cortex Moutan in serum metabolites 1H-NMR. Take NMR(NMR) technology on human serum test analysis, the application of partial least squares discriminant analysis(PLS-DA) metabolism spectral differences between in serum before and after taking the normal human Cortex Moutan charcoal, by comparing the metabolites differences.Found that normal human in serum before and after taking Cortex Moutan carbon present in the 1H-NMR peaks were significantly different, to provide a scientific basis moutan charcoal mechanism of action and clinical application in the human body.The fourth,Research on urine metabolomics Cortex Moutan hot charcoal on serum blood hemorrhage in rats.Taken orally ethanol / free drink hot blood caused by yeast combined subcutaneous hemorrhage rat model.Using serum and urine of rats were measured 1H-NMR analysis, select PLS-DA rats urine, in serum endogenous metabolites analyzed.Use Variable importance projection(VIP) and the correction rate of cooling bleeding charcoal filter out Cortex Moutan efficacy biomarkers, preliminary screening and blood heat hemorrhagic disease related biomarkers has one of the 3-hydroxybutyric acid, acetylsalicylic acid, creatine, lactic acid, alanine, taurine, glycine, choline, tryptophan, glucose, formic acid, tyrosine, Dimethyltryptamine, aspartic acid as the carbon cooling by bleeding Cortex Moutan pharmacodynamic biomarkers.Cortex Moutan charcoal can significantly affect blood heat bleeding rat urine, in serum metabolic effects of related substances, in order to further explain the mechanism of cooling blood to stop bleeding Cortex Moutan charcoal provided.