Study On The Tumor Inhibition Effects Of High Quality Quercetin Flavone Extracted From Ginkgo Biloba Leaves In Mice

Objective:The objectives of this paper are to observe the antitumor activity through the study of the The high flavonoid quercetin glycosides from the leaves of Ginkgo biloba injection(HQGBE injection) on tumor bearing mice(H22) tumor growth conditions and observe the of cyclophosphamide antitumor synergism through the HQGBE injection combined with cyclophosphamide in the growth of the tumor bearing mice and examine HQGBE injection on the immune effects through the detection plasma IL-2 and TNF-α concentration in the experimental mice.Methods:1 The inhibitory effects of HQGBE injection on tumor growth40 Kunming mice were selected and inoculated with hepatoma H22 tumor cells under the right forelimb armpit, randomly divided into experimental groups, namely,HQGBE injection 1.75mg/kg, 35 mg / kg, 70 mg / kg dose group, and cyclophosphamide(20 mg / kg) group and the control group. After 24 hours of incubation, the mice of the experimental group were given different doses of HQGBE injection, the model group given intraperitoneal injection of normal saline and the drug volume was 0.2ml/20g/d, daily one time for7 days. The mice were killed and the tumor blocks were stripped out and weighed to calculate the anti-tumor mice after the last administration of drugs for 24 hour.2 The inhibitory effects of low dose HQGBE injection combined with low dose cyclophosphamide on tumor growthThe mice were inoculated with hepatoma H22 tumor cells under the right forelimb armpit. They were randomly divided into 4 groups, the control group, HQGBE injection 17.5mg/kg dose group, cyclophosphamide group(5mg / kg) and the two-drugs-combined group(HQGBE injection 17.5mg/kg and cyclophosphamide 5mg / kg). After inoculation for 24 hours, the mice were given the medicines once a day for 7 days. Then, the animals were killed at the last administration of the drugs for 24 h, The tumor tissues were taken out and weighed to calculate the tumor inhibition mice.3 The effects of HQGBE injection on the plasma IL-2 and TNF-α in the experimental miceThe experimental mice were randomly divided into 5 groups: the model group, the HQGBE injection groups(3 different does groups), and cyclophosphamide injection group(20mg/kg) as the control group. After administration of the inoculation for 24 h, the mice were administered different drugs through intraperitoneal injection once a day for 7 days. The mice of the model control group were received the same volume of saline. After the last administration for 24 h, the blood samples were collected to determine the level of serum IL-2 and TNF-a concentration with ELISA assay.4 The effects on experimental mice by using HQGBE injection for 30 days20 Kunming mice were selected to inject 0.2ml tumor cell suspension through the right forelimb armpit. The mice were randomly divided into the model control group, HQGBE injection group(35mg/kg) After administration of the inoculation for 24 h, The treatment group was given HQGBE injection 0.2ml/20g/d(equivalent to Ginkgo biloba extract 35mg/kg), the model group received intraperitoneal injection of saline, once a day, for 30 days continuously. The animal death mice and general conditions were recorded. The experimental mice were killed after the last administration of the drugs. The tumor tissues were taken out and weighed to calculate the tumor inhibition mice.Results:1 The inhibitory effects of HQGBE injection on tumor growthThe results indicated that after administration of HQGBE injection for 7 days, the tumor tissue growth can be significantly inhibited in the experimental mice, and the tumor inhibition mice of 17.5mg/kg, 35mg/kg and 70mg/kg dose group were 22.6%, 48.8% and 40.2% respectively. The inhibition mice of cyclophosphamide group was 57.1%.2 The inhibitory effects of low dose HQGBE injection combined with cyclophosphamide on tumor growthThe results showed that tumor inhibition mice of HQGBE injection group(17.5mg/kg) and cyclophosphamide(5mg / kg) group were 18.1% and 23.9% respectively, the tumor inhibiting mice of the two-drug-combined group was 34.6%. Compared with the results of cyclophosphamide group, the inhibition mice was increased significantly.3 The effects of HQGBE injection on the concentration of serum plasma IL-2The results indicated that the level of serum IL-2 in HQGBE injection group( 35 mg / kg and group, 70 mg / kg) was significantly higher than that of model group(P < 0.01), and the level of serum IL-2 in the cyclophosphamide group was remarkably lower than that of the model group(P < 0.05);4. The effects of HQGBE injection on the concentration of serum plasma TNF-αThe results indicated that the level of serum TNF-α in HQGBE injection group( group35 mg / kg and group 70mg/kg) was significantly higher than that of model group(P< 0.01), and the level of serum TNF-α in the cyclophosphamide group was obviously lower than that of the control group(P < 0.05);5 The conditions of experimental mice after using of HQGBE injection for 30 daysAfter administrating HQGBE injection for 30 days, the results indicated that the weight of the mice in the model group was significantly reduced, the activities and their hair gloss became poor, their appetite was poor; however, the changes of body weight in the mice of HQGBE injection groups were not obvious, they had normal activities and normal hair luster; After administrating HQGBE injection for 30 days, the tumor inhibition effects were obvious, the average tumor weight of the model group were 3.862 g, but the average tumor weight of the mice in HQGBE injection group were 0.734 g. The tumor inhibition mice was 81%. The tumor blocks were observed and the tumor capsule was complete and the tumor tissue was liquefied remarkably. There was not obvious changes on the tumor liquefaction and the vessels in the tumor tissues were decreased significantly. The color of the tumor appeared dark red, tumor tissues were rich in blood vessels under microscope.Conclusion: The high quality of quercetin flavonoids from the leaves of Ginkgo biloba injection can inhibit the growth of the H22 tumor in mice, improve the general conditions of the tumor bearing mice and enhance the tumor inhibitory effects of cyclophosphamide potentiated antitumor effects; increase the levels of IL-2 and TNF-α in tumor bearing mice.