The Research Of Massa Medicata Fermentata ’s Quality Control By Chromatographic Fingerprint

Massa Medicata Fermentata(MMF) is a traditional fermented Chinese medicine,has a long history, and is one of the representatives of Chinese medicines component transformation. Massa Medicata Fermentata was recorded in “Yaoxinglun” of Early Tang Dynasty recorded.In the Han Dynasty, there had already recorded the recipes of Massa Medicata Fermentata made by natural fermentation with Artemisia annua, Polygonum, cocklebur grass, red bean, bitter almonds, flour and wheat bran with a certain proportion. The Massa Medicata Fermentata is general cube-shaped pieces, sallow face, rough, brittle, easily broken, slight aroma, and has the function of digestion. The Massa Medicata Fermentata has been widely used. The 2010 edition of "Chinese Pharmacopoeia" and "ministerial standards" had recorded 400-containing fermented Chinese medicine prescription preparations. A variety of commercially available Chinese patent medicines contain Massa Medicata Fermentata. But at present, the Massa Medicata Fermentata market is disordered, and the difference of quality is significant. The 2000 Pharmacopoeia recorded that Massa Medicata Fermentata was identified mainly by sensory experience and was lack of identification, and was also lack of the quality evaluation criteria. So it is urgent to build scientific effective quality evaluation standard of Massa Medicata Fermentata.This paper established the Massa Medicata Fermentata chromatographic fingerprint by the high performance liquid chromatography. In the paper, the effective components of the different origin of Massa Medicata Fermentata was extracted, the HPLC chromatographic conditions were established, the content of artemisinin, rutin and quercetin in the Massa Medicata Fermentata were determined, at the same time Massa Medicata Fermentata HPLC chromatographic fingerprint was established, and the five raw materials were also analyzed using the HPLC. The results are as follows: 1.Established the ectraction method of Massa Medicata FermentataThis paper compares the boiling method and ultrasonic method, boiling method is more in line with the concept of oral medicine, and more secure, better than the chromatogram boiling method results were determined using boiling water extraction method.2.Established the Massa Medicata Fermentata chromatographic fingerprint by the high performance liquid chromatographyHPLC Fingerprint instrument method for four different types of columns and conditions at different mobile phase ratio of exploration, and on the column temperature, velocity and wavelength of exploration, selection Acchrom XAqua C18 column elution, the establishment of : The mobile phase was 100% ultra-pure water, the column temperature 30 ℃, the injection volume was 10μL, a flow rate of 1m L / min, a wavelength of 280 nm.3. Determined Artemisinin,rutin and quercetin in Massa Medicata FermentataHPLC method is currently the principal component analysis method of traditional Chinese medicine, the method is simple, high sensitivity. Massa Medicata Fermentata as a raw material in major Chinese herbal medicine, artemisinin is the main component of Artemisia annua, rutin and quercetin is the main component of Polygonum. They are not only effective composition finished Massa Medicata Fermentata, but their content is also indirectly reflect the raw material Artemisia annua and Polygonum proportion accounted for. Artemisia annua and Polygonum proportion of Massa Medicata Fermentata has a dominant role in the quality of its finished products, artemisinin, rutin and quercetin in Massa Medicata Fermentata Massa Medicata Fermentata can be used as the basis of quality evaluation. Our results for the quality control and evaluation provides the basis for Massa Medicata Fermentata.4.Establish HPLC fingerprint of Massa Medicata FermentataThrough the establishment of eight different habitats Massa Medicata Fermentata chromatographic fingerprints for overall quality evaluation, through the "chromatographic fingerprint similarity evaluation system(2004A)" for analysis were obtained eight effective peak. By comparing the similarity of MMF differences across the overall quality of the Massa Medicata Fermentata, which Bozhou, Anhui and Sichuan Chengdu origin in the similarity of the two is closer than 0.8, and both product quality and origin have some protection. Workshop production of other origin are in the majority, coupled with the current country Massa Medicata Fermentata quality standards are not uniform, the formula is different. Environmental and production conditions throughout the fermentation have differences, resulting in around Massa Medicata Fermentata quality vary widely.