Research On Chemical Constituents And Pharmacological Activity Of Umhilicaria Esculenta

This chemical composition and antioxidant, antitumor,hypolipidemic activities of Umhilicaria esculenta on medicinal lichen body have been researched in this paper. At the same time research on iwhich inorganic elements were determined.Adopt detection technology of ICP-MS inorganic elements to determine the content of iron, copper, zinc, lead, arsenic, mercury, selenium, chromium, nickel, potassium, magnesium, aluminum, molybdenum, silver, scandium and cerium of U. esculenta were tested, Detected:Mn34.1mg/Kg、Fe 2110 mg/Kg、Cu 4.04mg/Kg、Zn 18.4mg/Kg、As 1.54mg/Kg、Hg 0.105mg/Kg、Se 1.15 mg/Kg、Pb 14.5mg/Kg、Cr 7.50 mg/Kg、Ni 2.24mg/Kg、K 1740mg/Kg、Mg 446mg/Kg、Al 2620mg/Kg、Mo 0.559mg/Kg、Ag 0.049mg/Kg、Sc 0.787 mg/Kg、Ce 6.89mg/Kg.The chemical composition,in the method of gradient extraction Umbilicaria be petroleum ether, chloroform, ethyl acetate, acetone, methanol extract, compounds were isolated and identified, they were ergosterol(1),Cis-hexane-1-3(2),Ergosterol peroxide(3), ethyl orsellinate(4), usnic acid(5), gyrophoric acid(6),orsellinic acid(7),ethyl everninate(8),Mannitol(9),Chlorophy(10).The Antioxidant activity, through determination the ability of ABTS+ to evaluate the antioxidant activity of orsellinic acid, gyrophoric acid, ethyl everninate in test concentration range,in which with the increase of molar concentration and ABTS+ free radical clearance rate,the three kinds of material of orsellinic acid, gyrophoric acid, ethyl everninate will increase.When the final concentration is 55μmol/L,the clearance rate of ABTS+ is 54.64%(P < 0.01),the concentrate of gyrophoric acid is 53.19%(P < 0.01), ethyl everninate is 46.33%(P<0.01).Inhibition of tumor, two monomer compounds with large amount are orsellinic acid, gyrophoric acid extracted from stone ear have been studied in this paper,which H22 has tumor-burdened tumor suppression effect on mice,and in order to illustrate the four types of compound effect on inhibition of tumor growth in mice,we test various indexes,for example the inhibitory rate, thymus index, spleen index and interleukin- 2 content and so on. The results show that orsellinic acid, high dose group of gyrophoric acid have good inhibitory effect on tumors in mice,and compared with negative control group significant difference(P < 0.01) have significant difference, the these groups of H22 tumor-burdened in mice serum and the content of Interleukin- 2 are increased significantly,which is associated with inhibiting tumor activity.Hypolipidemic activity, through the establishment of high fat diet induced hyperlipidemia model of different polar extracts of lichen and orsellinic acid, gyrophoric acid and the gradient extracts of Umhilicaria esculenta,we establishe the mice of hyperlipidemia model.The results show that the high and low dose group of orsellinic acid, gyrophoric acid,Methanol water extracts have significant hypolipidemic effect.