Curcumin Inhibites The Proliferation Of Human Lung Adenocarcinoma A549 Cell By Regulating MiR-21/PTEN Pathway

Purpose:curcumin,a natural phytochemical,has been shown to have potent anticancer activity.Here,we attempt to determine the molecular mechanisms of the cytotoxicity of curcumin against human non-small cell lung cancer cells.Methods : Human lung adenocarcinoma A549 cells were treated with different concentrations of curcumin for 48 h, The morphology and cell proliferation were observed under light microscope,and we use MTS assay to detect its effect on cell proliferation. The expression levels of micro RNA-21 in A549 cells which were treated with different concentrations of curcumin for 48 h were metasured by RT-q PCR. A549 cells were transfected with micro RNA-21 mimic or PTEN small interfering RNA(si RNA), causing mi R-21 overexpressed and PTEN gene silenced,its protein level of PTEN was determined by Western Blot after curcumin treatment.Results:Compared to the non-treated A549 cells, curcumin at 20-40μM can inhibit the proliferation of A549 cells in a dose-dependent way,and at the concentration of30μM suppressed the most significantlly(p<0.01). The expression level of micro RNA-21 in curcumin-treated A549 cells performed in a dose-dependent inhibition manner(p<0.05).Moreover,PTEN, a putative target of micro RNA-21,its protein level was detected by Western Blot. A549 cells transfected with mi R-21 mimics or PTEN si RNA, in contrast to the negative control group,PTEN protein levels were decreased(p<0.05),otherwise,to the untreated group, the PTEN protein level increased.Conclusion : Curcumin can significantly inhibite the proliferation of human lung adenocarcinoma A549 cells in a dose-dependent way.Mi R-21/PTEN pathway may be involved in the anti-cancer mechanisms of curcumin on NSCLC.