Expressions Of Toll Like Receptor 8(TLR8) And TLR9 In Lesions Of Condyloma Acuminatum

Objective: To explore the role of TLR8 and TLR9 on the pathogenesis of Condyloma acuminatum(CA) through detecting the expressions of TLR8 and TLR9 on skin lesions,and provide reference theory basis in the immunological pathogenesis of CA.Methods: An immunohistochemical method using Max vision(Ready-to-use immunohistochemical one-step quickly) was performed to detect the expression and distribution of TLR8 and TLR9 in specimens obtained from the CA lesions of 50 patients and from the foreskin of 10 normal human controls. And Image-Pro Plus 6.0(IPP6.0)color image analysis system for image analysis of quantitative research was applied. All measurement data are expressed by mean±standard.The results were analyzed by the SPSS 17.0 software.T test was used to compare cases and controls.The test results have statistical significance when P<0.05.Results: The first, Immunohistochemical localization analysis: TLR8, TLR9 protein was located in the cytoplasm and(or) membrane of keratinocytes. In normal skin samples, there was a weak expression of TLR8 in the stratum basale, but in the cytoplasm-based, a negative expression in keratinocytes of almost all epidermal layers except for stratum basale. In contrast, a strong expression of TLR8 in the stratum basale and stratum spinosum, but medium expression in the stratum corneum and stratum granulosum in CA lesions. In normal skin samples, TLR9 was strong positive expression in almost all epidermal layers except for stratum corneum, and in the CA lesions, TLR9 was strongly expressed in all epidermal layers, some particle aggregation. The second, IPP6.0 color image analysis of quantitative research: in the CA lesions, the relative amounts of protein expression of TLR8 and TLR9 are0.1267±0.05140 and 0.1604±0.06153, which are significantly higher than that in normal foreskins 0.0797±0.02805 and 0.1263±0.01724((all P<0.05).Conclusion: The expressions of TLR8 and TLR9 are markedly enhanced in CAlesions compared with normal skin,which may be served as recognition receptors for HPV infection and be involved in the body’s anti-HPV immune response.