The Repair Effect Of Wharton’s Jelly-derived Mesenchymal Stem Cells On Structure Of Oviducts Of New Zealand Rabbits With Chronic Salpingitis

Chapter1 The Model Establishment of Chronic Salpingitis of New Zealand Rabbits and The Therapeutic Action of Wharton’s Jelly-Derived Mesenchymal Stem Cells Objectives:To establish the model of chronic salpingitis of New Zealand Rabbits and to evaluated the therapeutic action of Wharton’s Jelly-Derived Mesenchymal Stem Cells with by HE staining.Method:(1) 30 clean New Zealand female Rabbits were devided into three groups, including 6rabbits in blank control groups, 12 rabbits in model control group and 12 rabbits in treatment group. Rabbits in model control group and treatment group were received vaginal administration by using escherichia coli.(2) Model control group was received two methods of treatment including intravenous+vaginal administration and vaginal administration by using sterilizing saline after 15 days of model establishment. They were marked as model A group and model B group respectively.(3) Treatment group was received two methods of treatment including intravenous+vaginal administration and vaginal administration by WJMSCs after 15 days of model establishment. They were marked as treatment A group and treatment B group respectively.(4) All the rabbits were humanely sacrifice after 7 days of the treatment of sterilizing saline or WJMSCs. Tissues of fallopian tube of each rabbit were selected, using for HE staining.Result:(1) Inflammatory response was obvious in the oviduct of model control group via surfaceobservation. Inflammatory response was alleviation in the oviduct of treatment model control group via surface observation.(2) Luminal stenosis, absence of mucosa, thickening of mesenchyme and muscular layer were observed in the oviduct of model control group via HE staining. This inflammatory tendency was improved after treatment of WJMSCs.Conclusion:(1) The model of chronic salpingitis of New Zealand Rabbits can be established via vaginal administration by using escherichia coli.(2) Recovery of structure of oviduct and level of inflammatory can be observed in surface observation and HE staining in the oviduct of treatment group after WJMSCs injection to New Zealand Rabbits.Chapter2 Analysis of The Repair of WJMSCs to Chronic Salpingitis via Stereology Objective:To measure the change of mucosal folds and muscular layer after Masson staining via stereology.Method:Structure of mucosal folds and muscular layer were compared between each group by measuring length of mucosal folds per unit area and volume density of muscular layer,on the basis of establishment of the model of chronic salpingitis of New Zealand Rabbits.Results:(1) The length of mucosal folds per unit area in model control group were significantly shorter than that in blank control group(P<0.05). The length of mucosal folds per unit area in both treatment A group and treatment B group were significantly longer than that in model control group(P<0.05; P<0.05). The length of mucosal folds per unit area in treatment B group were significantly longer than that in treatment B group(P<0.05).(2) The volume density of muscular layer in model control group was significantly higherthan that in blank control group(P<0.05). The volume density of muscular layer in treatment A group was significantly lower than that in model control group and treatment B group(P<0.05; P<0.05).Conclusion:(1) Chronic salpingitis can decrease the length of mucosal folds per unit area. After treatment of WJMSCs, the length of mucosal folds per unit area can increase. The therapeutic effect of vaginal administration was superior to that of intravenous+vaginal administration.(2) Chronic salpingitis can increase the volume density of muscular layer. After vaginal administration of WJMSCs, the volume density of muscular layer can restore.Chapter3 Analysis of The Relative Cytoskelatal Protein Via Real-time Quantitative Polymerase Chain Reaction Objective:To evaluate the change of m RNA expression of cytokeratin and vimentin via real-time quantitative polymerase chain reaction.Method:The m RNA expression of cytokeratin and vimentin were compared between each group via real-time quantitative polymerase chain reaction on the basis of establishment of the model of chronic salpingitis of New Zealand Rabbits.Results:(1) The m RNA expression of cytokeratin in model control group were significantly decrease than that in blank control group(P<0.05). The m RNA expression of cytokeratin in both treatment A group and treatment B group were significantly increase than that in model control group(P<0.05; P<0.05). The m RNA expression of cytokeratin in treatment B group were significantly increase than that in treatment B group(P<0.05).(2) The m RNA expression of vimentin in model control group was significantly increase than that in blank control group(P<0.05). The m RNA expression of vimentin bothtreatment A group and treatment B group were significantly decrease than that in model control group and treatment B group(P<0.05; P<0.05).Conclusion:(1) Chronic salpingitis can decrease the m RNA expression of cytokeratin. After treatment of WJMSCs, the m RNA expression of cytokeratin can increase.(2) Chronic salpingitis can increase the m RNA expression of vimentin. After treatment of WJMSCs, the m RNA expression of vimentin can increase.(3) The therapeutic effect of vaginal administration with 1×106 WJMSCs was superior to that of intravenous+vaginal administration.