Fibrinogen In Contrast-induced Nephropathy Model Early Changes And Significance Of Research

Objective:To build the mice contrast-induced nephropathy(CIN) model, to observe the renal pathological change in mice, mice serum creatinine(Scr) and Fibrinogen in urine(Fibrinogen, Fg) content, analyze urine Fibrinogen in contrast-induced nephropathy function and meaning of early renal damage. Methods:30 male C57 BL / 6 j mice, were randomly divided into blank control group(N), contrast media group(CM), kidney injury group(S), low dose contrast-induced nephropathy group(M1), high dose contrast-induced nephropathy group(M2), six in each group.The abdominal cavity, in turn, indomethacin injection interval of 15 minutes(INDO, 10 mg/kg), L- NG- nitro arginine methyl ester(L- NAME, 10 mg/kg), iodine, alcohol sand contrast agents(Iodixanol, 8.32 g I/kg, 10.40 g I/kg), contrast-induced nephropathy in mice model was established, to be put to death in mice after 24 hours.Then observe the mice kidney pathological changes.(2) ELISA to detect serum creatinine levels in mice.(3) ELISA to detect the contents of fibrinogen in urine in mice. Results:Compared with the N group, renal function, Scr,Fg in CM group no obvious change, S group and M1,M2 Scr and Fg significantly increases, the difference was statistically significant(P < 0.05);Compared with the CM group, group S and M1,M2, visible to the N group compared with the similar change;M1, M2 group compared with S group, Scr and Fg significantly increases, the difference was statistically significant(P< 0.05), while the Scr there was no statistically significant difference in M1 and M2 group(P> 0.05), and Fg in M1 and M2 group difference was statistically significant(P < 0.05). Conclusion:Urine fibrinogen in CIN kidney edema phase reversible phase can earlier hint of kidney damage in mice, the prevention and treatment of early diagnosis of CIN and subsequent have very positive meaning, therefore, we think the fibrinogen in the early diagnosis of CIN has potential application prospect.